完整叶绿体中单价和二价阳离子对于离子载体尼日利亚菌素和A23187作用的可用性。
Availability of monovalent and divalent cations within intact chloroplasts for the action of ionophores nigericin and A23187.
作者信息
Telfer A, Barber J, Nicolson J
出版信息
Biochim Biophys Acta. 1975 Aug 11;396(2):301-9. doi: 10.1016/0005-2728(75)90043-2.
PMID:808237
Abstract
- A23187 will uncouple electron transport by broken chloroplasts in a divalent cation dependent manner provided that they have been treated with a low concentration of EDTA. 2. A23187 stimulates oxaloacetate-dependent oxygen evolution and inhibits phosphoglycerate reduction by intact chloroplasts isolated in a cation-free medium whereas the full effect of nigericin was dependent on the presence of external K+. 3. Uncoupling of oxaloacetate reduction by A23187 in intact chloroplasts is inhibited by EDTA and this effect is overcome by excess Mg2+. 4. The results suggest that divalent and not monovalent cations are available for collapsing the light-induced H+ gradient within the intact organelle.
摘要
- 倘若用低浓度乙二胺四乙酸（EDTA）处理过，A23187会以二价阳离子依赖的方式使破碎的叶绿体中的电子传递解偶联。2. A23187刺激草酰乙酸依赖的氧气释放，并抑制在无阳离子培养基中分离出的完整叶绿体对磷酸甘油酸的还原，而尼日利亚菌素的全部作用则依赖于外部钾离子的存在。3. 在完整叶绿体中，A23187使草酰乙酸还原解偶联的作用受到EDTA抑制，而过量的镁离子可克服这种作用。4. 结果表明，在完整细胞器内，用于瓦解光诱导氢离子梯度的是二价阳离子而非一价阳离子。
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