Levels of cell wall enzymes in endospores and vegetative cells of Bacillus subtilis.
作者信息
Reusch V M, Hale S G, Hurly B J
出版信息
J Bacteriol. 1982 Dec;152(3):1147-53. doi: 10.1128/jb.152.3.1147-1153.1982.
Abstract
Vegetative bacilli and refractile endospores of Bacillus subtilis 168 were disrupted by homogenization with glass beads and fractionated by differential centrifugation. Most of the protein of endospores was particulate, whereas for bacilli most was soluble. Alanine racemase activity was sixfold higher in extract of endospores than in extract of bacilli and was particulate, whereas the enzyme from bacilli was soluble. The specific activities of seven other enzymes involved in peptidoglycan and teichoic acid biosynthesis were higher in extracts of bacilli than in those of endospores. The results suggest that restoration of activities of these seven enzymes to vegetative levels occurs during germination and outgrowth.
摘要
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本文引用的文献
1
Protein measurement with the Folin phenol reagent.
J Biol Chem. 1951 Nov;193(1):265-75.
2
The enzymatic synthesis of D-alanyl-D-alanine. I. Purification and properties of D-alanyl-D-alanine synthetase.
J Biol Chem. 1962 Mar;237:778-86.
3
4
THE SYNTHESIS OF TEICHOIC ACIDS. 3. GLUCOSYLATION OF POLYGLYCEROPHOSPHATE.
J Biol Chem. 1964 Oct;239:3187-91.
5
THE SYNTHESIS OF TEICHOIC ACIDS. I. POLYGLYCEROPHOSPHATE.
J Biol Chem. 1964 Oct;239:3168-77.
6
Pyrophosphorolysis and enzymic synthesis of cytidine diphosphate glycerol and cytidine diphosphate ribitol.
Biochem J. 1962 Feb;82(2):297-312. doi: 10.1042/bj0820297.
7
Teichoic and teichuronic acids: biosynthesis, assembly, and location.
Microbiol Rev. 1981 Jun;45(2):211-43. doi: 10.1128/mr.45.2.211-243.1981.
8
Biosythesis of the peptidoglycan of bacterial cell walls. II. Phospholipid carriers in the reaction sequence.
J Biol Chem. 1967 Jul 10;242(13):3180-90.
9
Biosynthesis of the peptidoglycan of bacterial cell walls. I. Utilization of uridine diphosphate acetylmuramyl pentapeptide and uridine diphosphate acetylglucosamine for peptidoglycan synthesis by particulate enzymes from Staphylococcus aureus and Micrococcus lysodeikticus.
Arch Biochem Biophys. 1966 Sep 26;116(1):487-515. doi: 10.1016/0003-9861(66)90056-7.
10
Cell wall polymers of Bacillus sphaericus 9602. II. Synthesis of the first enzyme unique to cortex synthesis during sporulation.
J Bacteriol. 1970 Aug;103(2):305-17. doi: 10.1128/jb.103.2.305-317.1970.
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