枯草芽孢杆菌芽孢和营养细胞中细胞壁酶的水平。
Levels of cell wall enzymes in endospores and vegetative cells of Bacillus subtilis.
作者信息
Reusch V M, Hale S G, Hurly B J
出版信息
J Bacteriol. 1982 Dec;152(3):1147-53. doi: 10.1128/jb.152.3.1147-1153.1982.
Abstract
Vegetative bacilli and refractile endospores of Bacillus subtilis 168 were disrupted by homogenization with glass beads and fractionated by differential centrifugation. Most of the protein of endospores was particulate, whereas for bacilli most was soluble. Alanine racemase activity was sixfold higher in extract of endospores than in extract of bacilli and was particulate, whereas the enzyme from bacilli was soluble. The specific activities of seven other enzymes involved in peptidoglycan and teichoic acid biosynthesis were higher in extracts of bacilli than in those of endospores. The results suggest that restoration of activities of these seven enzymes to vegetative levels occurs during germination and outgrowth.
摘要
枯草芽孢杆菌168的营养体杆菌和折光性芽孢通过玻璃珠匀浆破碎，并通过差速离心进行分级分离。芽孢的大部分蛋白质是颗粒状的，而杆菌的大部分蛋白质是可溶的。丙氨酸消旋酶活性在芽孢提取物中比在杆菌提取物中高六倍，并且是颗粒状的，而来自杆菌的酶是可溶的。参与肽聚糖和磷壁酸生物合成的其他七种酶的比活性在杆菌提取物中高于芽孢提取物。结果表明，在萌发和生长过程中，这七种酶的活性恢复到营养体水平。
相似文献
1
Levels of cell wall enzymes in endospores and vegetative cells of Bacillus subtilis.枯草芽孢杆菌芽孢和营养细胞中细胞壁酶的水平。
J Bacteriol. 1982 Dec;152(3):1147-53. doi: 10.1128/jb.152.3.1147-1153.1982.
2
Control of synthesis of wall teichoic acid during balanced growth of Bacillus subtilis W23.
J Gen Microbiol. 1982 Mar;128(3):593-9. doi: 10.1099/00221287-128-3-593.
3
Control of synthesis of wall teichoic acid in phosphate-starved cultures of Bacillus subtilis W23.枯草芽孢杆菌W23缺磷培养物中壁磷壁酸合成的调控
Eur J Biochem. 1981 Sep 1;118(3):497-500. doi: 10.1111/j.1432-1033.1981.tb05546.x.
4
CDP-glycerol:poly(glycerophosphate) glycerophosphotransferase, which is involved in the synthesis of the major wall teichoic acid in Bacillus subtilis 168, is encoded by tagF (rodC).CDP - 甘油：聚（甘油磷酸）甘油磷酸转移酶参与枯草芽孢杆菌168主要细胞壁磷壁酸的合成，由tagF（rodC）编码。
J Bacteriol. 1992 Jan;174(2):646-9. doi: 10.1128/jb.174.2.646-649.1992.
5
Biosynthesis of wall polymers in Bacillus subtilis.枯草芽孢杆菌中细胞壁聚合物的生物合成。
J Bacteriol. 1977 Jun;130(3):1055-63. doi: 10.1128/jb.130.3.1055-1063.1977.
6
Probing teichoic acid genetics with bioactive molecules reveals new interactions among diverse processes in bacterial cell wall biogenesis.利用生物活性分子探究磷壁酸遗传学揭示了细菌细胞壁生物合成中不同过程之间的新相互作用。
Chem Biol. 2009 May 29;16(5):548-56. doi: 10.1016/j.chembiol.2009.04.009.
7
The biosynthesis of wall teichoic acid by toluenised cells of Bacillus subtilis W23.枯草芽孢杆菌W23经甲苯处理的细胞合成壁磷壁酸的过程。
Eur J Biochem. 1981 Sep;119(1):85-90. doi: 10.1111/j.1432-1033.1981.tb05580.x.
8
Transcriptional control of peptidoglycan precursor synthesis during sporulation in Bacillus sphaericus.球形芽孢杆菌芽孢形成过程中肽聚糖前体合成的转录调控。
J Bacteriol. 1976 Feb;125(2):565-74. doi: 10.1128/jb.125.2.565-574.1976.
9
Regulation of the bacterial cell wall: analysis of a mutant of Bacillus subtilis defective in biosynthesis of teichoic acid.细菌细胞壁的调控：枯草芽孢杆菌磷壁酸生物合成缺陷突变体的分析
J Bacteriol. 1972 Apr;110(1):281-90. doi: 10.1128/jb.110.1.281-290.1972.
10
Localization and interactions of teichoic acid synthetic enzymes in Bacillus subtilis.枯草芽孢杆菌中磷壁酸合成酶的定位与相互作用
J Bacteriol. 2008 Mar;190(5):1812-21. doi: 10.1128/JB.01394-07. Epub 2007 Dec 21.
引用本文的文献
1
Harnessing β-Lactam Antibiotics for Illumination of the Activity of Penicillin-Binding Proteins in .利用β-内酰胺抗生素来照亮青霉素结合蛋白在 …… 中的活性。
ACS Chem Biol. 2020 May 15;15(5):1242-1251. doi: 10.1021/acschembio.9b00977. Epub 2020 Mar 20.
2
Use of the alr gene as a food-grade selection marker in lactic acid bacteria.将alr基因用作乳酸菌中的食品级选择标记。
Appl Environ Microbiol. 2002 Nov;68(11):5663-70. doi: 10.1128/AEM.68.11.5663-5670.2002.
3
Role and expression of the Bacillus subtilis rodC operon.枯草芽孢杆菌rodC操纵子的作用与表达
J Bacteriol. 1991 Jul;173(14):4341-6. doi: 10.1128/jb.173.14.4341-4346.1991.
本文引用的文献
1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
3
ENZYMATIC SYNTHESIS OF ANALOGS OF THE CELL-WALL PRECURSOR. I. KINETICS AND SPECIFICITY OF URIDINE DIPHOSPHO-N-ACETYLMURAMYL-L-ALANYL-D-GLUTAMYL-L-LYSINE:D-ALANYL-D-ALANINE LIGASE (ADENOSINE DIPHOSPHATE) FROM STREPTOCOCCUS FAECALIS R.细胞壁前体类似物的酶促合成。I. 粪链球菌R中尿苷二磷酸 - N - 乙酰胞壁酰 - L - 丙氨酰 - D - 谷氨酰 - L - 赖氨酸：D - 丙氨酰 - D - 丙氨酸连接酶（二磷酸腺苷）的动力学和特异性
Biochemistry. 1965 Jan;4:120-31. doi: 10.1021/bi00877a020.
4
THE SYNTHESIS OF TEICHOIC ACIDS. 3. GLUCOSYLATION OF POLYGLYCEROPHOSPHATE.磷壁酸的合成。3. 聚甘油磷酸的糖基化作用。
J Biol Chem. 1964 Oct;239:3187-91.
5
THE SYNTHESIS OF TEICHOIC ACIDS. I. POLYGLYCEROPHOSPHATE.磷壁酸的合成。I. 聚甘油磷酸
J Biol Chem. 1964 Oct;239:3168-77.
6
Pyrophosphorolysis and enzymic synthesis of cytidine diphosphate glycerol and cytidine diphosphate ribitol.焦磷酸解作用及二磷酸胞苷甘油和二磷酸胞苷核糖醇的酶促合成
Biochem J. 1962 Feb;82(2):297-312. doi: 10.1042/bj0820297.
7
Teichoic and teichuronic acids: biosynthesis, assembly, and location.磷壁酸和磷壁醛酸：生物合成、组装及定位
Microbiol Rev. 1981 Jun;45(2):211-43. doi: 10.1128/mr.45.2.211-243.1981.
8
Biosythesis of the peptidoglycan of bacterial cell walls. II. Phospholipid carriers in the reaction sequence.细菌细胞壁肽聚糖的生物合成。II. 反应序列中的磷脂载体。
J Biol Chem. 1967 Jul 10;242(13):3180-90.
9
Biosynthesis of the peptidoglycan of bacterial cell walls. I. Utilization of uridine diphosphate acetylmuramyl pentapeptide and uridine diphosphate acetylglucosamine for peptidoglycan synthesis by particulate enzymes from Staphylococcus aureus and Micrococcus lysodeikticus.细菌细胞壁肽聚糖的生物合成。I. 金黄色葡萄球菌和溶壁微球菌的颗粒酶利用尿苷二磷酸乙酰胞壁酰五肽和尿苷二磷酸乙酰葡糖胺进行肽聚糖合成。
Arch Biochem Biophys. 1966 Sep 26;116(1):487-515. doi: 10.1016/0003-9861(66)90056-7.
10
Cell wall polymers of Bacillus sphaericus 9602. II. Synthesis of the first enzyme unique to cortex synthesis during sporulation.球形芽孢杆菌9602的细胞壁聚合物。II. 芽孢形成过程中皮层合成特有的第一种酶的合成。
J Bacteriol. 1970 Aug;103(2):305-17. doi: 10.1128/jb.103.2.305-317.1970.
Zotero 插件