枯草芽孢杆菌中细胞壁聚合物的生物合成。
Biosynthesis of wall polymers in Bacillus subtilis.
作者信息
Wyke A W, Ward J B
出版信息
J Bacteriol. 1977 Jun;130(3):1055-63. doi: 10.1128/jb.130.3.1055-1063.1977.
Abstract
Preparations of membrane plus wall derived from Bacillus subtilis W23 were used to study the in vitro synthesis of peptidoglycan and teichoic acid and their linkage to the preexisting cell wall. The teichoic acid synthesis showed an ordered requirement for the incorporation of N-acetylglucosamine from uridine 5'-diphosphate (UDP)-N-acetylglucosamine followed by addition of glycerol phosphate from cytidine 5'-diphosphate (CDP)-glycerol and finally by addition of ribitol phosphate from CDP-ribitol. UDP-N-acetylglucosamine was not only required for the synthesis of the teichoic acid, but N-acetylglucosamine residues formed an integral part of the linkage unit attaching polyribitol phosphate to the cell wall. Synthesis of the teichoic acid was exquisitely sensitive to the antibiotic tunicamycin, and this was shown to be due to the inhibition of incorporation of N-acetylglucosamine units from UDP-N-acetylglucosamine.
摘要
使用源自枯草芽孢杆菌W23的细胞膜加细胞壁制剂来研究肽聚糖和磷壁酸的体外合成及其与预先存在的细胞壁的连接。磷壁酸合成显示出对从尿苷5'-二磷酸（UDP）-N-乙酰葡糖胺掺入N-乙酰葡糖胺的有序需求，随后从胞苷5'-二磷酸（CDP）-甘油添加甘油磷酸，最后从CDP-核糖醇添加核糖醇磷酸。UDP-N-乙酰葡糖胺不仅是磷壁酸合成所必需的，而且N-乙酰葡糖胺残基是将多核糖醇磷酸连接到细胞壁的连接单元的组成部分。磷壁酸的合成对抗生素衣霉素极为敏感，这表明是由于抑制了UDP-N-乙酰葡糖胺中N-乙酰葡糖胺单元的掺入。
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