DeBerardinis M, Wargin W A
J Chromatogr. 1982 Sep 3;246(1):89-94. doi: 10.1016/s0021-9673(00)82785-5.
A simple and sensitive method for the simultaneous analysis of carbaryl and 1-naphthol in whole blood by reversed-phase high-performance liquid chromatography and fluorescence detection is described. Spiked blood (heparinized) containing an internal standard was hemolyzed and extracted with ethyl acetate. After centrifugation the extractant was removed and taken to dryness. Reconstitution and subsequent high-performance liquid chromatography-fluorescence analysis yielded linear standard curves for carbaryl and 1-naphthol. Linear response vs. concentration profiles were obtained for carbaryl and 1-naphthol extracted from buffer solutions as well. A simple chemical hydrolysis study of carbaryl is included to illustrate the effectiveness of the extraction procedure and assay.
本文描述了一种采用反相高效液相色谱法和荧光检测法同时分析全血中甲萘威和1-萘酚的简单灵敏方法。将添加了内标的加标血液（肝素化）进行溶血处理，并用乙酸乙酯萃取。离心后去除萃取剂并使其干燥。复溶并随后进行高效液相色谱-荧光分析，得到了甲萘威和1-萘酚的线性标准曲线。从缓冲溶液中萃取的甲萘威和1-萘酚也获得了线性响应与浓度曲线。文中还包括一项甲萘威的简单化学水解研究，以说明萃取程序和分析方法的有效性。
