Nicholson G A, Sugars J
J Neurol Sci. 1982 Oct;56(1):11-6. doi: 10.1016/0022-510x(82)90056-9.
A number of recent reports suggest that intracellular calcium concentration is increased in skeletal muscle in duchenne muscular dystrophy (DMD). Leucocyte chemiluminescent responses are dependent on calcium ions and can be induced by the calcium ionophore A23187. Chemiluminescence may therefore reflect intracellular calcium levels. In order to determine whether non-muscle cells in DMD share the calcium abnormality, we have examined A23187-induced chemiluminescence in DMD leucocytes. Peak calcium dependent chemiluminescence occurred at 0.25-0.75 mM added calcium chloride. Peak chemiluminescent responses in the 13-paired samples were reduced in DMD leucocytes (P less than 0.01) using a paired t-test. These result suggest that there is an abnormality of calcium dependent A23187-induced chemiluminescence in DMD leucocytes and that there may be a generalised abnormality of calcium metabolism in DMD.
最近的一些报告表明，杜氏肌营养不良症（DMD）患者骨骼肌中的细胞内钙浓度会升高。白细胞化学发光反应依赖于钙离子，并且可由钙离子载体A23187诱导。因此，化学发光可能反映细胞内钙水平。为了确定DMD中的非肌肉细胞是否存在钙异常，我们检测了DMD白细胞中A23187诱导的化学发光。添加氯化钙时，钙依赖性化学发光峰值出现在0.25 - 0.75 mM。使用配对t检验，13对样本中DMD白细胞的化学发光峰值反应降低（P小于0.01）。这些结果表明，DMD白细胞中存在钙依赖性A23187诱导的化学发光异常，并且DMD中可能存在普遍的钙代谢异常。
