Singh M P, Sharan M, Verma S B
J Biomed Eng. 1982 Oct;4(4):279-84. doi: 10.1016/0141-5425(82)90044-9.
A study of the simultaneous diffusion of O2 and CO2 in the presence of haemoglobin is made by considering the intermediate compound hypothesis of Adair. Our analysis exhibits explicit dependence of the fractional saturation (of haemoglobin with O2) on pCO2. It has been emphasized that discrepancies in the various numerically computed values of equilibrium constants arising in the oxygen dissociation curve may be due to inadequacy of the methods used to solve the normal equations obtained in the process of least-square curve-fitting, which are highly non-linear in character in the present context. It has been shown, by using the method suggested by Marquardt that the values of these constants lead to a saturation function which fits very well with the one based on documented data. The analysis further shows that under normal physiological conditions, the main bulk of oxygenated haemoglobin exists in the fully saturated form as HbO8 which also seems obvious from our computed values of equilibrium constants.
通过考虑阿代尔的中间化合物假说，对血红蛋白存在下氧气和二氧化碳的同时扩散进行了研究。我们的分析表明，（血红蛋白与氧气的）分数饱和度明确依赖于二氧化碳分压。需要强调的是，氧解离曲线中出现的各种平衡常数数值计算结果存在差异，可能是由于用于求解最小二乘曲线拟合过程中得到的正规方程的方法不够充分，而在当前情况下这些方程具有高度非线性特征。利用马夸特提出的方法表明，这些常数的值导致一个饱和函数，该函数与基于文献数据的饱和函数拟合得非常好。分析还表明，在正常生理条件下，大部分氧合血红蛋白以完全饱和的形式HbO₈存在，从我们计算的平衡常数数值来看这似乎也很明显。
