de Castro J M, Paullin S K, DeLugas G M
J Comp Physiol Psychol. 1978 Jun;92(3):571-9. doi: 10.1037/h0077485.
Seven adult male rats were observed for body weight and microregulation (feeding, drinking, and running patterns) after manipulation of insulin and glucagon levels. They received three injections per day for 3 days each week of 3 U of protamine zinc insulin, .25 mg of zinc glucagon, 50 microgram of protamine zinc somatostatin (SRIF), or protamine zinc vehicle. Diabetes was then induced with an iv injection of streptozotocin (65 mg/kg), and the injection schedule was repeated after the full diabetic syndrome emerged. In all rats whose insulin levels were increased relative to glucagon levels, body weight increased; in those whose glucagon levels were increased relative to insulin levels, body weight decreased. All injections except vehicle reduced meal sizes in both normal and diabetic rats, but only insulin increased the frequency of feeding. These effects could be predicted by the glucostatic theory of food intake regulation and are thus interpreted as supportive of this theory. These results also support the hypothesis that the relative concentration of insulin to glucagon is a regulator of body weight set point.
对七只成年雄性大鼠在胰岛素和胰高血糖素水平被调控后观察其体重及微量调节（进食、饮水和奔跑模式）。它们每周三天，每天接受三次注射，分别注射3单位的精蛋白锌胰岛素、0.25毫克的锌胰高血糖素、50微克的精蛋白锌生长抑素（SRIF）或精蛋白锌赋形剂。然后通过静脉注射链脲佐菌素（65毫克/千克）诱导糖尿病，在完全出现糖尿病综合征后重复注射方案。在所有胰岛素水平相对于胰高血糖素水平升高的大鼠中，体重增加；在那些胰高血糖素水平相对于胰岛素水平升高的大鼠中，体重下降。除赋形剂外的所有注射均减少了正常和糖尿病大鼠的进食量，但只有胰岛素增加了进食频率。这些效应可以通过食物摄入调节的糖稳态理论来预测，因此被解释为支持该理论。这些结果也支持了胰岛素与胰高血糖素的相对浓度是体重设定点调节因子的假说。
