[哺乳动物的糖苷酶：某些疾病中活性与水平变化的关联]

[Glycosidases of mammals: association of activities and changes of levels in some disorders].

作者信息

Calvo P, Reglero A, Pérez N, Martín J, Llanillo M, Serrano M A, García M V, Rodríguez J A, Rodríguez F J, Carretero M I, Barba J L, Sánchez C, Cabezas J A

出版信息

Rev Esp Fisiol. 1982;38 Suppl:211-6.

PMID:6815736

Abstract

beta-Galactosidase and associated activities (beta-glucosidase and beta-fucosidase) have been studied in rabbit and bovine liver and rabbit spleen. The physico-chemical (optimal pH, pI, MW) and kinetical (Km, Vmax, Ki) properties were determined for all the activities. Two enzyme forms were separated in rabbit spleen. beta-Galactosidase, beta-fucosidase and beta-glucosidase activities were catalyzed by the same enzyme in rabbit and bovine liver. The enzyme from bovine liver showed nonlinear double-reciprocal plots, suggesting a substrate-activation model, and the presence of more than one binding site in the enzyme. The enzyme activities of several glycosidases were determined in human sera fom control groups and from patients with diabetes mellitus, pancreatitis, hepatitis, cirrhosis, stomach and breast cancer, myocardial infarction and renal failure. The results show significantly different enzyme levels for several glycosidases in all the studied diseases. Experimentally-induced diabetes mellitus, alcoholism and nephrotoxicity in rats showed different glycosidase levels in several tissues, as compared with control groups.

摘要

已对兔和牛的肝脏以及兔的脾脏中的β-半乳糖苷酶及相关活性（β-葡萄糖苷酶和β-岩藻糖苷酶）进行了研究。测定了所有活性的物理化学性质（最适pH、pI、分子量）和动力学性质（Km、Vmax、Ki）。在兔脾脏中分离出了两种酶形式。兔和牛肝脏中的β-半乳糖苷酶、β-岩藻糖苷酶和β-葡萄糖苷酶活性由同一种酶催化。牛肝脏中的酶呈现非线性双倒数图，提示存在底物激活模型，且该酶中存在不止一个结合位点。测定了对照组以及患有糖尿病、胰腺炎、肝炎、肝硬化、胃癌和乳腺癌、心肌梗死及肾衰竭的患者血清中几种糖苷酶的酶活性。结果表明，在所有研究的疾病中，几种糖苷酶的酶水平存在显著差异。与对照组相比，实验诱导的大鼠糖尿病、酒精中毒和肾毒性在多个组织中呈现出不同的糖苷酶水平。