Ziska P, Kindt A, Franz H
Acta Histochem. 1982;71(1):29-33. doi: 10.1016/s0065-1281(82)80008-1.
A lectin has been isolated from extracts of garden cress (Lepidium sativum) by affinity chromatography on human immunoglobulin-Sepharose. The lectin reacts with human erythrocytes without specificity for the A, B and 0 blood group. Erythrocytes of animal origin are also agglutinated by the lectin. The hemagglutinating activity is abolished by heating the lectin solution at 70 degrees C or by dialysis against strong acid buffers. The hemagglutination reaction is not inhibited by monosaccharides. Lectin-glycoprotein interactions are described and discussed.
通过在人免疫球蛋白-琼脂糖凝胶上进行亲和层析，从水田芥（独行菜）提取物中分离出一种凝集素。该凝集素与人红细胞发生反应，对A、B和O血型无特异性。动物源红细胞也会被该凝集素凝集。通过将凝集素溶液在70℃加热或用强酸缓冲液透析，可消除其血凝活性。单糖不会抑制血凝反应。对凝集素-糖蛋白相互作用进行了描述和讨论。
