Kolberg J
Acta Pathol Microbiol Scand C. 1978 Jun;86C(3):99-104. doi: 10.1111/j.1699-0463.1978.tb02565.x.
A mitogenic lectin has been isolated from saline extract of Lathyrus odoratus seeds by (NH4)2SO4 precipitation and subsequent chromatography on DEAE-Sepharose and CM-Sepharose. The isolated lectin was almost homogeneous by SDS gel electrophoresis and gel filtration, but multiple bands were obtained on isoelectric focusing. The molecular weight, determined by gel filtration, was 37,000. Gel electrophoresis in the presence of SDS gave a molecular weight of 19,000, thus suggesting that the lectin is a dimer. It agglutinated human erythrocytes of the different ABO groups equally well. Human lymphocytes were exposed to the lectin or PHA, and the incorporation of thymidine was measured. Compared with PHA, which stimulated maximally in the concentration range of 0.25--1microgram/ml, the L. odoratus lectin had optimal activity at concentrations of 35 microgram/ml and higher.
通过硫酸铵沉淀以及随后在DEAE-琼脂糖和CM-琼脂糖上进行色谱分离，从香豌豆种子的盐提取物中分离出一种促有丝分裂凝集素。通过SDS凝胶电泳和凝胶过滤，分离出的凝集素几乎是均一的，但在等电聚焦时获得了多条带。通过凝胶过滤测定的分子量为37,000。在SDS存在下的凝胶电泳给出的分子量为19,000，因此表明该凝集素是二聚体。它对不同ABO血型组的人红细胞凝集效果相同。将人淋巴细胞暴露于该凝集素或PHA，并测量胸苷的掺入情况。与在0.25 - 1微克/毫升浓度范围内刺激最大的PHA相比，香豌豆凝集素在35微克/毫升及更高浓度下具有最佳活性。
