猪组织蛋白质合成范围的研究：甲状腺激素的作用。

Studies on the range of tissue protein synthesis in pigs: the effect of thyroid hormones.

作者信息

Simon O, Bergner H, Münchmeyer R, Zebrowska T

出版信息

Br J Nutr. 1982 Nov;48(3):571-82. doi: 10.1079/bjn19820142.

DOI:10.1079/bjn19820142

Abstract

The effects of thyroid hormones on the range of tissue protein synthesis in growing pigs using the constant infusion technique with [14C]lysine were studied. 2. During a 6 h infusion, samples were taken from blood and, at the end of the infusion, from liver, pancreas, stomach, small and large intestines, kidney cortex, kidney medulla, muscle and skin. 3. Lower relative specific radioactivities of free leucine and lysine in several tissues were observed in the hormone-treated group than in the untreated one. 4. The range of protein synthesis rate and the daily amount of protein synthesized in tissues was higher in all tissues after application of thyroid hormones. 5. Assuming that the organs analysed represented 70% of the total trichloroacetic acid-precipitable protein of the pig, the estimated range of daily protein synthesis was 251-490 and 312-880 g in untreated and hormone-treated pigs respectively.

摘要

利用[14C]赖氨酸恒速输注技术，研究了甲状腺激素对生长猪组织蛋白质合成范围的影响。2. 在6小时的输注过程中，从血液中取样，输注结束时，从肝脏、胰腺、胃、小肠和大肠、肾皮质、肾髓质、肌肉和皮肤取样。3. 激素处理组中几个组织中游离亮氨酸和赖氨酸的相对比放射性低于未处理组。4. 应用甲状腺激素后，所有组织中的蛋白质合成速率范围和每日合成的蛋白质量均更高。5. 假设所分析的器官占猪总三氯乙酸沉淀蛋白的70%，未处理猪和激素处理猪的每日蛋白质合成估计范围分别为251 - 490克和312 - 880克。