Archer I M, Harper P S, Wusteman F S
Biochim Biophys Acta. 1982 Nov 9;708(2):134-40. doi: 10.1016/0167-4838(82)90213-8.
Iduronate 2-sulphate sulphatase (EC 3.1.6.-) was found in human placenta in three forms which could be separated by elution from DEAE-cellulose using an NaCl gradient. Form C, most firmly bound to DEAE-cellulose, was 40% larger than the other two (forms A and B in order of ease of elution from the ion exchanger). Forms B and C contained sialic acid which could be removed by neuraminidase digestion. After removal of sialic acid form B became indistinguishable from form A. The enzyme forms found in placenta were compared with those from other human tissues and fluids by means of DEAE-cellulose chromatography and gel chromatography. Serum and amniotic fluid contained only form C, urine and cultured fibroblasts contained the less-anionic forms as well, and kidney contained appreciable amounts only of form A. Pre- and post-natal diagnosis of the Hunter syndrome both involve measurements on the enzyme which is present in form C. This is not accompanied by less-anionic forms which constitute the bulk of the enzyme as it is isolated from easily available sources such as urine.
艾杜糖醛酸2-硫酸酯酶（EC 3.1.6.-）在人胎盘中以三种形式存在，可通过使用NaCl梯度从DEAE-纤维素上洗脱来分离。形式C与DEAE-纤维素结合最紧密，比其他两种形式（按从离子交换剂上洗脱的难易程度依次为形式A和形式B）大40%。形式B和形式C含有唾液酸，可通过神经氨酸酶消化去除。去除唾液酸后，形式B与形式A无法区分。通过DEAE-纤维素色谱法和凝胶色谱法将胎盘中发现的酶形式与其他人体组织和体液中的酶形式进行了比较。血清和羊水仅含有形式C，尿液和培养的成纤维细胞也含有阴离子较少的形式，而肾脏仅含有相当数量的形式A。亨特综合征的产前和产后诊断都涉及对以形式C存在的酶的测量。这与从尿液等容易获得的来源分离的酶中占大部分的阴离子较少的形式不同。
