一种新型猪脑垂体前叶多肽的分离及氨基末端序列。与胰岛素原、促胰液素和劳氏肉瘤病毒转化蛋白TVFV60的同源性。
Isolation and NH2-terminal sequence of a novel porcine anterior pituitary polypeptide. Homology to proinsulin, secretin and Rous sarcoma virus transforming protein TVFV60.
作者信息
Hsi K L, Seidah N G, De Serres G, Chrétien M
出版信息
FEBS Lett. 1982 Oct 18;147(2):261-6. doi: 10.1016/0014-5793(82)81055-7.
An Mr 21 000 polypeptide, designated APPG, has been purified by reverse-phase, high-performance liquid chromatography (RP-HPLC), from acid extracts of porcine anterior pituitary glands. This acidic protein possesses an isoelectric point of 4.9. Amino acid analysis shows that it is not a glycoprotein and estimates it to contain about 173 amino acids. NH2-terminal sequence analysis allowed the determination of the first 50 residues unambiguously. A computer data bank search using a mutation data matrix and comparison with 269 012 protein segments indicated that this is a novel polypeptide sequence. However, this search revealed suggestive sequence homologies to a number of peptides of known sequence, including duck proinsulin (30%), Rous sarcoma virus transforming protein TVFV60 (24%) and pig secretin (26%).
一种分子量为21000的多肽，命名为APPG，已通过反相高效液相色谱（RP-HPLC）从猪垂体前叶的酸性提取物中纯化出来。这种酸性蛋白的等电点为4.9。氨基酸分析表明它不是糖蛋白，估计含有约173个氨基酸。氨基末端序列分析明确确定了前50个残基。使用突变数据矩阵进行的计算机数据库搜索以及与269012个蛋白质片段的比较表明，这是一个新的多肽序列。然而，该搜索揭示了与许多已知序列的肽存在暗示性的序列同源性，包括鸭胰岛素原（30%）、劳氏肉瘤病毒转化蛋白TVFV60（24%）和猪促胰液素（26%）。
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