通过X射线微量分析研究EDTA处理对人类精子亚细胞区域锌的去除作用。

Removal of zinc from subcellular regions of human spermatozoa by EDTA treatment studied by X-ray microanalysis.

作者信息

Roomans G M, Lundevall E, Björndahl L, Kvist U

出版信息

Int J Androl. 1982 Oct;5(5):478-86. doi: 10.1111/j.1365-2605.1982.tb00279.x.

DOI:10.1111/j.1365-2605.1982.tb00279.x

Abstract

The distribution of zinc in the head, neck and mid-piece of human ejaculated spermatozoa was investigated with energy-dispersive X-ray microanalysis. EDTA treatment within 1 h after ejaculation caused removal of 80-90% of the zinc in these regions. Storage for 24 h in own seminal plasma did not alter sperm zinc content. However, these aged spermatozoa only lost 35% of their zinc content on EDTA treatment. The stronger binding of zinc, occurring upon storage in seminal plasma, developed in all 3 parts of the cell investigated. These results are discussed in the light of the role of zinc in nuclear chromatin decondensation ability and sperm head-tail connection.

摘要

采用能量色散X射线微量分析技术，研究了锌在人类射出精子头部、颈部和中段的分布情况。射精后1小时内用乙二胺四乙酸（EDTA）处理，可使这些区域80 - 90%的锌去除。在自身精浆中储存24小时不会改变精子锌含量。然而，这些老化精子经EDTA处理后仅损失35%的锌含量。在精浆中储存时发生的锌更强结合，在所研究细胞的所有三个部分都有出现。根据锌在核染色质解聚能力和精子头尾连接中的作用对这些结果进行了讨论。