Syuto B, Kubo S
Mol Cell Biochem. 1982 Oct 1;48(1):25-32. doi: 10.1007/BF00214819.
The purification and crystallization of type C botulinum toxin along with its physical characteristics are described. The shape of Clostridium botulinum type C toxin molecule is globular like a pressed ball with a 7.4 nm diameter and a 4.3 nm thickness. The molecular volume is approximately 185 nl and the molecular weight is 141000. The toxin molecule is composed of two parts, which are separable under appropriate conditions. These parts have some differences in the electrophoretic properties, amino acid distribution, immunological, and functional characteristics. The toxin molecule can be reconstituted by association of S-S bond between the two chains. The expression of the toxicity requires that the fragments of the polypeptide chain carrying the necessary information be functionally organized for the proper development of the specific tertiary structure for active conformation.
描述了C型肉毒杆菌毒素的纯化、结晶及其物理特性。C型肉毒杆菌毒素分子的形状呈球状，像一个压扁的球，直径为7.4纳米，厚度为4.3纳米。分子体积约为185纳升，分子量为141000。毒素分子由两部分组成，在适当条件下可分离。这些部分在电泳特性、氨基酸分布、免疫学和功能特性方面存在一些差异。毒素分子可通过两条链之间的S-S键结合而重组。毒性的表达要求携带必要信息的多肽链片段在功能上进行组织，以形成特定的三级结构以获得活性构象。
