Boroff D A, Dasgupta B R, Fleck U S
J Bacteriol. 1968 May;95(5):1738-44. doi: 10.1128/jb.95.5.1738-1744.1968.
Gerwing et al. described the isolation and purification from culture filtrates of the toxin of Clostridium botulinum type B and characterized it as a homogeneous protein of less than 10,000 molecular weight. Analysis by various methods of samples of this toxin obtained from Gerwing et al., and preparations produced by their methods in our laboratories, furnished convincing evidence that neither her preparation nor ours was homogeneous. The molecular weight of the toxic component isolated from either of the preparations was 100,000 or greater and resembled, in a number of respects, the alpha component isolated by us from the crystalline toxin of C. botulinum type A.
格温等人描述了从肉毒梭菌B型毒素的培养滤液中进行分离和纯化，并将其鉴定为一种分子量小于10,000的均质蛋白质。通过各种方法对从格温等人获得的这种毒素样本以及我们实验室用他们的方法制备的样本进行分析，提供了令人信服的证据，表明她的制备物和我们的制备物都不是均质的。从任何一种制备物中分离出的有毒成分的分子量为100,000或更大，并且在许多方面类似于我们从肉毒梭菌A型结晶毒素中分离出的α成分。
