Ohishi I, Iwasaki M, Sakaguchi G
Infect Immun. 1981 Mar;31(3):890-5. doi: 10.1128/iai.31.3.890-895.1981.
Botulinum C2 toxin has vascular permeability as well as lethal activities. Both activities are elicited by cooperation of two dissimilar protein components, designated components I and II, which individually have very low activities. The vascular permeability activity of C2 toxin, demonstrated as blueing response after intravenous injection of Evans blue, was markedly enhanced by treatment with trypsin and was abolished by neutralization with either anti-component I or II serum. Inflammatory reactions, such as edema, congestion, and hemorrhage, were found at the site of intradermal injection of trypsinized C2 toxin. No vascular permeability activity was demonstrated by the intradermal injection of the toxin of Clostridium botulinum types A through F. These results indicate that C2 toxin has a novel biological activity, which is not possessed by the neurotoxin elaborated by C. botulinum types A through F. This suggests that C2 toxin causes lethality in a different way from that of botulinum neurotoxin, which is known to inhibit the presynaptic release of acetylcholine at the neuromuscular junction.
肉毒杆菌C2毒素具有血管通透性及致死活性。这两种活性均由两种不同的蛋白质成分协同引发，分别称为成分I和成分II，它们单独的活性都非常低。通过静脉注射伊文思蓝后呈现的发蓝反应来证明的C2毒素的血管通透性活性，经胰蛋白酶处理后显著增强，并用抗成分I或II血清中和后则被消除。在皮内注射经胰蛋白酶处理的C2毒素的部位发现了炎症反应，如水肿、充血和出血。皮内注射A型至F型肉毒杆菌毒素未显示血管通透性活性。这些结果表明，C2毒素具有一种新型生物活性，这是A型至F型肉毒杆菌产生的神经毒素所不具备的。这表明C2毒素导致致死的方式与肉毒杆菌神经毒素不同，已知后者可抑制神经肌肉接头处乙酰胆碱的突触前释放。
