Allison J P, Belvedere M, Reisfeld R A, Pellegrino M A, Ferrone S
J Immunol. 1978 Aug;121(2):579-85.
The specificity of A9 antiserum produced in rabbits immunized with high density lipoprotein-associated HLA-A9 from serum was investigated by using serologic and immunochemical approaches. After suitable absorption, the xenoantiserum No. 2958 is monospecific for A9 antigens in direct cytotoxic test. Data from lysostrip experiments and from blocking of cytotoxicity with Fab2 fragments indicate that the A9 xenoantiserum No. 2958 react with the same or closely associated antigenic structures recognized by A9 alloantisera. Immunoprecipitates obtained with the specific xenoantiserum No. 2958 and analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis contained only proteins of the size of detergent-solubilized HLA and beta2-microglobulin (45,000 and 12,000 m.w.). Immunodepletion experiments indicated that the precipitation was mediated by antibodies directed to the heavy chain, and not the light chain of the complex. These results indicate that monospecific HLA-A9 xenoantisera have the same discriminatory capacity of alloantisera and can be useful for the serologic and immunochemical characterization of HLA antigens.
采用血清学和免疫化学方法，研究了用血清中高密度脂蛋白相关的HLA - A9免疫兔所产生的A9抗血清的特异性。经过适当吸收后，异种抗血清2958在直接细胞毒性试验中对A9抗原具有单特异性。溶菌条实验以及用Fab2片段阻断细胞毒性的数据表明，A9异种抗血清2958与A9同种抗血清识别的相同或密切相关的抗原结构发生反应。用特异性异种抗血清2958获得的免疫沉淀物经十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳分析，仅含有去污剂可溶解的HLA和β2 - 微球蛋白大小的蛋白质（分子量分别为45,000和12,000）。免疫去除实验表明，沉淀是由针对复合物重链而非轻链的抗体介导的。这些结果表明，单特异性HLA - A9异种抗血清具有与同种抗血清相同的鉴别能力，可用于HLA抗原的血清学和免疫化学鉴定。
