一种用于检测针对细胞表面抗原的细胞毒性抗体的高灵敏度且可重复的微量细胞毒性试验。

A highly sensitive and reproducible microcytotoxicity assay for demonstrating cytotoxic antibodies to cell surface antigens.

作者信息

Hellström I, Brown J P, Klitzman J M, Hellström K E

出版信息

J Immunol Methods. 1978;22(3-4):369-81. doi: 10.1016/0022-1759(78)90044-3.

Abstract

An assay is described, in which target cells are suspended in diluted rabbit serum (as a source of complement) and pipetted into the wells of Falcon No. 3034 Microtest plates, to which diluted antiserum (or control serum) has been added. After 18--24 h of incubation, the plates are stained with crystal violet and counted using an electronic Quantimet image analyzer. This technique could reproducibly detect complement-dependent cytotoxic antibodies to target cell H-2 antigens and to MuLV gp70 on cell lines which expressed this antigen, at serum dilutions up to 1:160,000. The cytotoxic antibodies could be removed by absorption of sera with cells carrying the respective antigens.

摘要

本文描述了一种检测方法，将靶细胞悬浮于稀释的兔血清（作为补体来源）中，用移液器吸取后加入已添加稀释抗血清（或对照血清）的Falcon No. 3034微量测试板孔中。孵育18 - 24小时后，用结晶紫对平板进行染色，并使用电子定量图像分析仪进行计数。该技术能够在血清稀释至1:160,000时，可重复地检测针对表达该抗原的细胞系上靶细胞H-2抗原和MuLV gp70的补体依赖性细胞毒性抗体。细胞毒性抗体可通过用携带相应抗原的细胞吸收血清而去除。