Mazabraud A
Biochimie. 1982 Oct;64(10):955-60. doi: 10.1016/s0300-9084(82)80359-3.
The nucleotide sequence of Xenopus laevis phenylalanine tRNA extracted from oocytes was determined to be: pGCCGAAAUAm2GCUCm1AG DDGGGAGAGCm22 G psi psi AGACmUGmAAYA psi C UAAAGm7GDCm5CCUGGT psi CGm1AUCCCGG GUUUCGGCACCAoH. This result was achieved by analysing, with classical procedures [6], the oligonucleotides obtained after digestion by T1 or pancreatic ribonuclease. This sequence is identical to the mammalian sequence. It has been entirely conserved during 10(8) years, the time lapse between the divergence of amphibians and mammals in evolution. In contrast to 5S RNA, no important heterogeneity has been found in the oocyte sequence, suggesting that there is only a single sequence for tRNAphe in X. laevis. Small differences are seen in the elution pattern from RPC-5 columns for immature oocyte and somatic tRNAphe. They are probably due to a submodification of methyl-5-cytidine residues, which appear to be about half methylated in tRNAphe as well as in total tRNA from immature oocytes.
从非洲爪蟾卵母细胞中提取的苯丙氨酸tRNA的核苷酸序列测定结果如下：pGCCGAAAUAm2GCUCm1AG DDGGGAGAGCm22 G ψ ψ AGACmUGmAAYA ψ C UAAAGm7GDCm5CCUGGT ψ CGm1AUCCCGG GUUUCGGCACCAoH。该结果是通过采用经典方法[6]分析经T1或胰核糖核酸酶消化后得到的寡核苷酸而获得的。此序列与哺乳动物的序列相同。在两栖动物和哺乳动物在进化中分化后的10^8年时间里，该序列完全保守。与5S RNA不同，在卵母细胞序列中未发现重要的异质性，这表明非洲爪蟾的苯丙氨酸tRNA只有单一序列。未成熟卵母细胞的苯丙氨酸tRNA和体细胞的苯丙氨酸tRNA在RPC - 5柱上的洗脱模式存在微小差异。它们可能是由于5 - 甲基胞嘧啶残基的亚修饰所致，在苯丙氨酸tRNA以及未成熟卵母细胞的总tRNA中，这些残基似乎约有一半被甲基化。
