Shea J W, Huber G L, Holmes L, Homans A
J Lab Clin Med. 1978 Aug;92(2):270-82.
The in vitro antibacterial activity of rat alveolar macrophages against a challenge of radiolabeled Staphylococcus epidermidis was studied following 30 and 60 consecutive days of in vivo tobacco smoke inhalation in a dose equivalent to approximately 1 1/2 packs of unfiltered cigarettes per day in man. Macrophages harvested by bronchopulmonary lavage, were cultured, infected, and assayed hourly for 3 hr to determine the relative percentage of surviving radiolabeled intracellular bacteria. Macrophages harvested from smoke-treated rats for 30 days had an impaired capacity to kill bacteria when compared to macrophages from control and sham-smoked animals (63.5% +/- 3.3, 80.8% +/- 3.8, and 86.2% %/- 1.2 killed in 3 hr, respectively). Alveolar macrophages harvested from rats exposed to cigarette smoke for 60 days, however, did not have an impairment in their bactericidal activity relative to matched controls (73.3% +/- 3.9 vs 69.8% +/- 6.9 killed in 3 hr). These data imply that doses of cigarette smoke commonly consumed by man impair the in vitro bactericidal function of the rat alveolar macrophages following 30 consecutive days of experimental smoke treatment. The disappearance of this effect after 60 days of exposure to tobacco smoke suggests that an adaptation to the initial impairment has developed.
在体内连续30天和60天吸入相当于人类每天约1.5包未过滤香烟剂量的烟草烟雾后，研究了大鼠肺泡巨噬细胞对放射性标记的表皮葡萄球菌攻击的体外抗菌活性。通过支气管肺灌洗收集巨噬细胞，进行培养、感染，并每小时检测3小时，以确定存活的放射性标记细胞内细菌的相对百分比。与来自对照和假吸烟动物的巨噬细胞相比，从接受30天烟雾处理的大鼠中收获的巨噬细胞杀灭细菌的能力受损（分别在3小时内杀灭63.5%±3.3%、80.8%±3.8%和86.2%±1.2%）。然而，从暴露于香烟烟雾60天的大鼠中收获的肺泡巨噬细胞，相对于匹配的对照，其杀菌活性没有受损（3小时内杀灭73.3%±3.9%对69.8%±6.9%）。这些数据表明，在连续30天的实验性烟雾处理后，人类通常吸入的香烟烟雾剂量会损害大鼠肺泡巨噬细胞的体外杀菌功能。暴露于烟草烟雾60天后这种效应的消失表明已经形成了对初始损伤的适应性。
