Gilmore D S, Schick D G, Montgomerie J Z
J Clin Microbiol. 1982 Nov;16(5):865-7. doi: 10.1128/jcm.16.5.865-867.1982.
Pseudomonas aeruginosa colonization is found in a high percentage of males with spinal cord injury. The perineum is the body site most frequently colonized, and specific serotypes may persist for weeks. We examined patients for the presence of P. aeruginosa and Klebsiella pneumoniae on the perineum and adjacent body sites by using contact plates. P. aeruginosa, K. pneumoniae, or both were cultured from perineal swabs of 22 male patients. Wells (2.5 cm2) containing agar medium selective for these organisms were used to examine samples from 32 sites adjacent to the perineum in each patient. P. aeruginosa was most frequently cultured from samples taken from the perineum, the scrotum, and the penile shaft. K. pneumoniae isolation was more variable; this organism was most commonly found on the perineum and scrotum. Rectal swabs, obtained through a proctoscope, were positive for P. aeruginosa in four of eight patients with this organism on the perineum and positive for K. pneumoniae in eight of nine patients with this organism on the perineum. These studies more clearly define the extent of the colonization of the perineum and adjacent body sites which provides potential reservoirs of P. aeruginosa and K. pneumoniae.
脊髓损伤男性患者中铜绿假单胞菌定植率很高。会阴部是最常被定植的身体部位，特定血清型可能会持续数周。我们通过使用接触平板检查患者会阴部及相邻身体部位是否存在铜绿假单胞菌和肺炎克雷伯菌。从22例男性患者的会阴部拭子中培养出了铜绿假单胞菌、肺炎克雷伯菌或两者皆有。使用含有对这些微生物有选择性的琼脂培养基的孔（2.5平方厘米）来检查每位患者会阴部相邻的32个部位的样本。铜绿假单胞菌最常从会阴部、阴囊和阴茎体采集的样本中培养出来。肺炎克雷伯菌的分离情况更具变异性；该菌最常见于会阴部和阴囊。通过直肠镜获取的直肠拭子，在会阴部有该菌的8例患者中有4例铜绿假单胞菌呈阳性，在会阴部有该菌的9例患者中有8例肺炎克雷伯菌呈阳性。这些研究更清楚地界定了会阴部及相邻身体部位的定植范围，这些部位是铜绿假单胞菌和肺炎克雷伯菌的潜在储存库。
