Foote R H, Ladd P C, Lafaunce N A, McCauley A D, Hasler J F
J Dairy Sci. 1982 Nov;65(11):2164-9. doi: 10.3168/jds.S0022-0302(82)82476-4.
Six Holstein cows in a commercial herd (three superovulated and three controls) and eight Holstein cows superovulated a total of 12 times in an experimental herd were studied. Superovulation was induced primarily by treatment with follicle stimulating hormone and prostaglandin F2 alpha. Milk was weighed twice daily for 30 days following treatment in the commercial herd with no effect on production. Milk samples were saved on Monday, Wednesday, and Friday to determine progesterone content. Last milk at mid-cycle averaged 8.2 ng/ml of progesterone for the cows in the experimental herd, and 7 days after superovulation they averaged 52.6 +/- 10.2 ng/ml (mean +/- standard error) of progesterone. The correlation with number of embryos recovered was .86. Therefore, milk progesterone may be useful in monitoring superovulatory response. Also, injection of prostaglandin F2 alpha into superovulated cows 9 days after a previous injection did not initiate a new estrous cycle, a fact accurately monitored by milk progesterone determinations.
对一个商业牛群中的6头荷斯坦奶牛（3头进行了超数排卵，3头作为对照）以及一个实验牛群中8头共进行了12次超数排卵的荷斯坦奶牛进行了研究。超数排卵主要通过促卵泡激素和前列腺素F2α处理诱导。在商业牛群中处理后，每天对牛奶称重两次，持续30天，对产奶量没有影响。在周一、周三和周五采集牛奶样本以测定孕酮含量。实验牛群中的奶牛在周期中期的末次牛奶中孕酮平均含量为8.2纳克/毫升，超数排卵7天后平均为52.6±10.2纳克/毫升（均值±标准误）。其与回收胚胎数量的相关性为0.86。因此，牛奶孕酮可能有助于监测超数排卵反应。此外，在先前注射前列腺素F2α 9天后对超数排卵奶牛再次注射，并未引发新的发情周期，这一情况通过牛奶孕酮测定得以准确监测。
