5-羟色胺对大鼠牙髓外植体前列腺素E2生物合成的体外刺激作用。

Stimulation of prostaglandin E2 biosynthesis in rat dental pulp explants in vitro by 5-hydroxytryptamine.

作者信息

Hirafuji M, Terashima K, Satoh S, Ogura Y

出版信息

Arch Oral Biol. 1982;27(11):961-4. doi: 10.1016/0003-9969(82)90103-0.

Abstract

When excised rat dental pulp tissue was incubated in Krebs solution, prostaglandin E2 (PGE2) was released into the medium. The release was completely blocked by the PG synthetase inhibitor, indomethacin (0.1 mM). Exogenous arachidonic acid (0.01 mM), a substrate of PG synthetase, increased the amount of PGE2 production and the endogenous PGE2 biosynthesis was strongly stimulated by 5-hydroxytryptamine (5-HT) at concentrations higher than 0.01 mM. Bradykinin (0.1 mM) and histamine (0.1 mM) had no effect.

摘要

当将切除的大鼠牙髓组织在 Krebs 溶液中孵育时，前列腺素 E2（PGE2）会释放到培养基中。这种释放被 PG 合成酶抑制剂吲哚美辛（0.1 mM）完全阻断。外源性花生四烯酸（0.01 mM）是 PG 合成酶的底物，可增加 PGE2 的产生量，并且在浓度高于 0.01 mM 时，5-羟色胺（5-HT）强烈刺激内源性 PGE2 的生物合成。缓激肽（0.1 mM）和组胺（0.1 mM）没有作用。