Alemany S, Varela I, Mato J M
Biochem J. 1982 Nov 15;208(2):453-7. doi: 10.1042/bj2080453.
The addition of 1 microM-vasopressin or -angiotensin to isolated rat hepatocytes induced a fast transient inhibition of the rate of incorporation of [Me-3H]choline into phosphatidylcholine. The cationophore A23187 induced a similar inhibition of phosphatidylcholine synthesis. The addition of micromolar Ca2+ to rat liver microsomes inhibited the activity of CDP-choline: 1,2-diacylglycerol cholinephosphotransferase. This inhibition is due a decrease in the Vmax. of the enzyme without affecting the Km for CDP-choline. It is concluded that Ca2+ regulates phosphatidylcholine synthesis in rat liver.
向分离的大鼠肝细胞中添加1微摩尔的血管加压素或血管紧张素会快速短暂抑制[甲基 - ³H]胆碱掺入磷脂酰胆碱的速率。离子载体A23187也会诱导类似的磷脂酰胆碱合成抑制。向大鼠肝脏微粒体中添加微摩尔浓度的钙离子会抑制CDP - 胆碱：1,2 - 二酰基甘油胆碱磷酸转移酶的活性。这种抑制是由于该酶的最大反应速度（Vmax）降低，而不影响其对CDP - 胆碱的米氏常数（Km）。得出的结论是钙离子调节大鼠肝脏中的磷脂酰胆碱合成。
