Hughes B P, Rye K A, Pickford L B, Barritt G J, Chalmers A H
Biochem J. 1984 Sep 1;222(2):535-40. doi: 10.1042/bj2220535.
Vasopressin induced a transient increase of 50% in the total concentration of diacylglycerols (determined by g.l.c.) in isolated hepatocytes. The increase was maximal at 0.25 min, and the concentration of diacylglycerols in cells treated with vasopressin had returned to the basal value by 4 min. No change in the concentration of diacylglycerols was observed after the treatment of cells with glucagon. The dependency of this effect on the concentration of vasopressin was similar to that of the effect of the hormone on 45Ca2+ efflux measured at 0.1 mM extracellular Ca2+. Vasopressin increased the proportion of arachidonic acid and stearic acid and decreased the proportion of oleic acid present in the diacylglycerols. In hepatocytes prelabelled with [14C]arachidonic acid, vasopressin increased the amount of [14C]diacylglycerol. The effects of vasopressin on the total concentration of diacylglycerols and [14C]diacylglycerol were mimicked by an exogenous phospholipid phosphodiesterase (phospholipase C) from Clostridium perfringens. The results are consistent with the conclusion that the transient increase in diacylglycerols induced by vasopressin is caused by the rapid hydrolysis of both the phosphoinositides and one or more other phospholipids.
血管加压素可使离体肝细胞中甘油二酯的总浓度（通过气相色谱法测定）瞬间增加50%。这种增加在0.25分钟时达到最大值，用血管加压素处理的细胞中甘油二酯的浓度在4分钟时已恢复到基础值。用胰高血糖素处理细胞后，未观察到甘油二酯浓度的变化。这种效应对血管加压素浓度的依赖性与该激素对在0.1 mM细胞外钙离子浓度下测量的45Ca2+外流的效应相似。血管加压素增加了甘油二酯中花生四烯酸和硬脂酸的比例，并降低了油酸的比例。在用[14C]花生四烯酸预标记的肝细胞中，血管加压素增加了[14C]甘油二酯的量。产气荚膜梭菌的外源性磷脂磷酸二酯酶（磷脂酶C）模拟了血管加压素对甘油二酯总浓度和[14C]甘油二酯的影响。结果与以下结论一致，即血管加压素诱导的甘油二酯瞬间增加是由磷酸肌醇和一种或多种其他磷脂的快速水解引起的。
