Shen-Ong G L, Keath E J, Piccoli S P, Cole M D
Cell. 1982 Dec;31(2 Pt 1):443-52. doi: 10.1016/0092-8674(82)90137-4.
We have found that the myc oncogene has been modified by abortive recombination with the alpha heavy-chain immunoglobulin constant-region (C alpha) gene in five different mouse plasmacytoma lines. Recombination occurred approximately 0.8-2.0 kb to the 5' side of two distinct coding regions, defined by sequence homology between the chicken cellular and plasmacytoma myc genes. The myc and C alpha genes were always in opposite transcriptional orientation, with the recombination site within the C alpha switch region sequences. DNA recombination was found to correlate with the production of a novel 2.1 kb species of myc RNA that was 0.4 kb shorter than the normal cellular transcript. No elevated levels of myc RNA were evident, suggesting that DNA rearrangements have altered the myc oncogene product. This oncogene activation corresponds to the chromosomal translocations found in nearly all plasmacytomas.
我们发现，在五种不同的小鼠浆细胞瘤细胞系中，myc癌基因已通过与α重链免疫球蛋白恒定区（Cα）基因的无效重组而发生改变。重组发生在两个不同编码区5'端约0.8 - 2.0 kb处，这两个编码区由鸡细胞和浆细胞瘤myc基因之间的序列同源性定义。myc和Cα基因总是处于相反的转录方向，重组位点位于Cα转换区序列内。发现DNA重组与一种新的2.1 kb的myc RNA的产生相关，该RNA比正常细胞转录本短0.4 kb。未发现myc RNA水平升高，这表明DNA重排改变了myc癌基因产物。这种癌基因激活与几乎所有浆细胞瘤中发现的染色体易位相对应。
