Harris L J, Remmers E F, Brodeur P, Riblet R, D'Eustachio P, Marcu K B
Nucleic Acids Res. 1983 Dec 10;11(23):8303-15. doi: 10.1093/nar/11.23.8303.
In murine plasmacytomas, the c-myc gene has frequently been found to undergo rearrangement by virtue of a T(12;15) chromosome translocation. The immunoglobulin heavy chain gene switch region (S alpha) constitutes the target for most of these recombinations particularly in IgA producing plasmacytomas. We sought to identify non-S alpha myc target sites in several IgG producing tumors. The c-myc target in MPC-11 (a BALB/c IgG2b producing plasmacytoma) has been cloned, localized to the Igh-C locus and identified as the gamma 2a heavy chain gene switch region (S gamma 2a). Furthermore, by Southern blot hybridization, we have determined that the S gamma 2b region is the c-myc target in two NZB IgG2b producing plasmacytomas. The potential relation between Ig class expressed and c-myc translocation target is discussed.
在鼠浆细胞瘤中，经常发现c-myc基因因T(12;15)染色体易位而发生重排。免疫球蛋白重链基因转换区（Sα）是这些重组中大多数的靶点，特别是在产生IgA的浆细胞瘤中。我们试图在几种产生IgG的肿瘤中鉴定非Sα myc靶点。MPC-11（一种产生BALB/c IgG2b的浆细胞瘤）中的c-myc靶点已被克隆，定位于Igh-C基因座，并被鉴定为γ2a重链基因转换区（Sγ2a）。此外，通过Southern印迹杂交，我们确定Sγ2b区域是两种产生NZB IgG2b的浆细胞瘤中的c-myc靶点。讨论了所表达的Ig类别与c-myc易位靶点之间的潜在关系。
