Rabinovitch A, Alejandro R, Noel J, Brunschwig J P, Ryan U S
Diabetes. 1982 Aug;31 Suppl 4:48-54. doi: 10.2337/diab.31.4.s48.
Prolongation of rat pancreatic islet allograft survival by a prior 7-day period of tissue culture was demonstrated, confirming previous reports by others. We then sought to identity those cells in islets capable of stimulating allograft rejection (Ia antigen-bearing cells) and to determine whether such cells and/or their Ia antigens might be reduced by tissue culture. Freshly isolated and 7-day-cultured Wistar-Furth rat islets were incubated with a mouse anti-rat Ia nonpolymorphic monoclonal antibody, then with peroxidase-conjugated goat anti-mouse antibody, and processed for electron microscopy. Peroxidase (Ia)-positive lymphocytes, macrophages, and capillary endothelial cells were identified in fresh but not in cultured islets. A radioligand assay, using 125I-protein A, revealed a 45% decrease in binding of Ia antibody to cultured compared with fresh islet cells. We conclude that Ia antigen-bearing lymphocytes, macrophages, and capillary endothelial cells in rat islets are reduced by tissue culture and that this may account, at least in part, for the decreased immunogenicity of cultured islet allografts.
先前7天的组织培养可延长大鼠胰岛同种异体移植的存活时间，这一结果得到了证实，与其他人之前的报告一致。然后，我们试图鉴定胰岛中那些能够刺激同种异体移植排斥反应的细胞（携带Ia抗原的细胞），并确定这种细胞和/或其Ia抗原是否会因组织培养而减少。将新鲜分离的和培养7天的Wistar-Furth大鼠胰岛与小鼠抗大鼠Ia非多态性单克隆抗体孵育，然后与过氧化物酶偶联的山羊抗小鼠抗体孵育，并进行电子显微镜处理。在新鲜胰岛中鉴定出了过氧化物酶（Ia）阳性淋巴细胞、巨噬细胞和毛细血管内皮细胞，但在培养的胰岛中未鉴定出。使用125I-蛋白A的放射性配体分析显示，与新鲜胰岛细胞相比，Ia抗体与培养胰岛细胞的结合减少了45%。我们得出结论，大鼠胰岛中携带Ia抗原的淋巴细胞、巨噬细胞和毛细血管内皮细胞因组织培养而减少，这可能至少部分解释了培养胰岛同种异体移植免疫原性降低的原因。
