Weintraub H, Buscaglia M, Ferrez M, Weiller S, Boulet A, Fabre F, Baulieu E E
C R Seances Acad Sci III. 1982 Dec 20;295(13):787-90.
Microinjection of a crude yeast extract into fully grown immature Xenopus laevis oocytes induces the reinitiation of meiotic maturation as does Xenopus laevis oocyte MPF (Maturation Promoting Factor) by itself. Using several strains of yeast exhibiting temperature sensitive cell cycle block (cdc), it was observed that yeast extract exhibits MPF activity only when obtained during the time interval from the G2 period to the metaphase. This observation stresses eucaryotic ubiquity of MPF.
将粗制酵母提取物显微注射到完全成熟的未成熟非洲爪蟾卵母细胞中，会诱导减数分裂成熟的重新启动，非洲爪蟾卵母细胞促成熟因子（MPF）自身也会如此。使用几种表现出温度敏感型细胞周期阻滞（cdc）的酵母菌株，观察到酵母提取物仅在从G2期到中期的时间间隔内获得时才表现出MPF活性。这一观察结果强调了MPF在真核生物中的普遍性。
