Morisaki N, Stitts J M, Bartels-Tomei L, Milo G E, Panganamala R V, Cornwell D G
Artery. 1982;11(2):88-107.
Smooth muscle cells from guinea pig aorta were grown in tissue culture. Dipyridamole enhanced the proliferation of these cells in culture and dipyridamole overcame the inhibitory effect of arachidonic acid on cell proliferation. Dipyridamole and the antioxidant vitamin E both increased the cloning potential and the number of population doublings for smooth muscle cells in culture. Lipid peroxidation was measured in cultured cells with thiobarbituric acid. Dipyridamole, vitamin E and butylated hydroxytoluene inhibited lipid peroxidation both in cultures treated with media alone and in cultures treated with arachidonic acid. Dipyridamole enhanced PGI2 biosynthesis while vitamin E and butylated hydroxytoluene had no effect on PGI2 biosynthesis. These data show that cell proliferation is related to lipid peroxidation rather than PGI2 biosynthesis. Dipyridamole functions as an antioxidant that stimulates the proliferation of aorta smooth muscle cells.
将豚鼠主动脉平滑肌细胞进行组织培养。双嘧达莫可促进这些培养细胞的增殖，并且双嘧达莫可克服花生四烯酸对细胞增殖的抑制作用。双嘧达莫和抗氧化剂维生素E均可增加培养的平滑肌细胞的克隆潜力和群体倍增数。用硫代巴比妥酸测定培养细胞中的脂质过氧化作用。双嘧达莫、维生素E和丁基羟基甲苯在仅用培养基处理的培养物以及用花生四烯酸处理的培养物中均抑制脂质过氧化作用。双嘧达莫可增强前列环素（PGI2）的生物合成，而维生素E和丁基羟基甲苯对PGI2的生物合成无影响。这些数据表明，细胞增殖与脂质过氧化作用而非PGI2生物合成有关。双嘧达莫作为一种抗氧化剂，可刺激主动脉平滑肌细胞的增殖。
