Barriga O O
J Parasitol. 1978 Aug;64(4):638-44.
Spleen cells from mice pretreated with a Trichinella spiralis extract (TsE-mice) showed severe depression of the response to lipopolysaccharide (LPS) and to concanavalin A (Con A), slight depression to phytohemagglutinin (PHA) and normal response to tuberculin purified protein derivative (PPD) as compared to saline-pretreated controls. Mice pretreated with bovine serum albumin (BSA-mice) revealed greatly reduced responses to LPS, somewhat reduced response to Con A, and normal responses to PHA and to PPD. Only TsE-mice showed significant reduction in the number of rosette-forming cells and of direct and indirect plaque-forming cells (DPFC and IPFC). BSA-mice exhibited some reduction of the DPFC only. Direct hemagglutinating (HA) titers were equivalent in the 3 groups after immunization with sheep erythrocytes but facilitated HA titers were depressed in TsE-mice. The total number and the number of viable cells were similar in the spleens of all animals. TsE treatment causes a reduction in the number of T1 lymphocytes and an inhibition of the late differentiation of B cells in the spleen. Suppressor T-cells apparently play a major but not exclusive role in T. spiralis-induced nonspecific immunodepression.
与用生理盐水预处理的对照组相比，用旋毛虫提取物预处理的小鼠（旋毛虫提取物预处理小鼠）的脾细胞对脂多糖（LPS）和刀豆球蛋白A（Con A）的反应严重降低，对植物血凝素（PHA）的反应略有降低，对结核菌素纯蛋白衍生物（PPD）的反应正常。用牛血清白蛋白预处理的小鼠（牛血清白蛋白预处理小鼠）对LPS的反应大大降低，对Con A的反应有所降低，对PHA和PPD的反应正常。只有旋毛虫提取物预处理小鼠的玫瑰花结形成细胞以及直接和间接空斑形成细胞（DPFC和IPFC）数量显著减少。牛血清白蛋白预处理小鼠仅表现出DPFC有所减少。用绵羊红细胞免疫后，三组的直接血凝（HA）滴度相当，但旋毛虫提取物预处理小鼠的促血凝滴度降低。所有动物脾脏中的细胞总数和活细胞数相似。旋毛虫提取物处理导致脾脏中T1淋巴细胞数量减少以及B细胞晚期分化受到抑制。抑制性T细胞显然在旋毛虫诱导的非特异性免疫抑制中起主要但非唯一作用。
