Dubey J P, Mehlhorn H
J Parasitol. 1978 Aug;64(4):689-95.
The development of Isospora ohioensis was studied in mice by feeding tissues of mice inoculated with oocysts to coccidia-free dogs and by the examination of mesenteric lymph nodes using light and electron microscopes. Extraintestinal organs of mice became infectious to dogs within 1 day after ingesting oocysts and remained infectious for at least 211 days after inoculation (DAI). Isospora ohioensis sporozoites were found in lymphoreticular cells of mesenteric lymph nodes of mice from 1-374 DAI. Intracellular sporozoites were located in parasitophorous vacuoles. Sporozoites grew from 5--6 to 11--16 micron in length on the 39th DAI but never lost the 2 crytalloid bodies typical for coccidian sporozoites. PAS-positive granules accumulated gradually in intracellular sporozoites with duration of infection in mice. The appearance of parasitophorous vacuoles varied with duration of infection. Beginning with 7 DAI, the vacuole contained a marginal zone of electron-dense material (up to 0.8 micron wide), giving the appearance of a cyst wall or sheath under the light microscope; a true cyst wall was was not found.
通过将接种卵囊的小鼠组织喂给无球虫的犬，并使用光学显微镜和电子显微镜检查肠系膜淋巴结，对俄亥俄等孢球虫在小鼠体内的发育进行了研究。小鼠的肠外器官在摄入卵囊后1天内对犬具有感染性，并且在接种后至少211天保持感染性（接种后天数，DAI）。在接种后天数1至374天的小鼠肠系膜淋巴结的淋巴网状细胞中发现了俄亥俄等孢球虫的子孢子。细胞内子孢子位于寄生泡内。子孢子在接种后天数39天时长度从5 - 6微米增长到11 - 16微米，但从未失去球虫属子孢子典型的2个晶体状小体。随着在小鼠体内感染时间的延长，细胞内子孢子中PAS阳性颗粒逐渐积累。寄生泡的外观随感染时间而变化。从接种后天数7天开始，泡内含有一个电子致密物质的边缘区（宽度达0.8微米），在光学显微镜下呈现出囊壁或鞘的外观；未发现真正的囊壁。
