Mehlhorn H, Markus M B
Z Parasitenkd. 1976 Dec 30;51(1):15-24. doi: 10.1007/BF00380524.
Stages of Isospora felis of the cat in the mesenteric lymph node of the mouse 25 days after oral inoculation with oocysts, have been described at the ultrastructural level. The organisms occurred singly within parasitophorous vacuoles in host cell cytoplasm and were sporozoite-like, having a large crystalloid body up to 5.5 mum in length posterior to the nucleus. The size and appearance of the parasitophorous vacuole varied. Some vacuoles contained numerous, small, electron dense granules about 30 nm in diameter. Because of the aggregation of granules and their arrangement within the parasitophorous vacuole, the impression was sometimes gained by light microscopy that parasites were surrounded by a sheath or cyst wall. However, a cyst wall was not present. In host cells, spherical, membrane-bound bodies with a homogeneous, electron dense core and a maximum diameter of 0.25 mum were filed along the limiting membrane of the parasitophorous vacuole. These extra-intestinal parasites were considered to be waiting stages, with a biological function similar to that of the tissue cyst stage of other general of isosporan coccidia.
在给小鼠经口接种卵囊25天后，在小鼠肠系膜淋巴结中，对猫等孢球虫的各阶段进行了超微结构水平的描述。虫体单个存在于宿主细胞质的寄生泡内，呈子孢子样，在细胞核后方有一个长达5.5μm的大晶体。寄生泡的大小和外观各不相同。一些泡内含有许多直径约30nm的小电子致密颗粒。由于颗粒的聚集及其在寄生泡内的排列，有时在光学显微镜下会产生寄生虫被鞘或囊壁包围的印象。然而，并不存在囊壁。在宿主细胞中，沿寄生泡的界膜排列着球形、膜结合体，其核心均匀、电子致密，最大直径为0.25μm。这些肠外寄生虫被认为是等待阶段，其生物学功能与其他等孢球虫属的组织囊肿阶段相似。
