Reddy C A, Cornell C P
J Gen Microbiol. 1982 Dec;128(12):2851-5. doi: 10.1099/00221287-128-12-2851.
Growth and acid metabolic products were similar when Corynebacterium pyogenes was grown aerobically or anaerobically in a serum-free medium (SFM). This indicated that C. pyogenes obtains energy for growth primarily by fermentative metabolism even under aerobic growth conditions. Growth yield was reduced by 90% in SFM minus glucose, 50% in SFM minus NaHCO3, 90% in SFM minus yeast extract, 100% in SFM minus Trypticase and yeast extract, and 30% in SFM minus haemin or Trypticase. Growth was not detectable when a known mixture of amino acids, vitamins, and nucleic acid bases were substituted for Trypticase and yeast extract in SFM; addition to the latter medium of a peptide source such as Trypticase or casitone supported good growth of the organism. When NaHCO3 was omitted from SFM and dissolved CO2 in the medium was rigorously excluded, growth was undetectable indicating that C. pyogenes has an obligate requirement for CO2 for growth. Succinate, formate and acetate were the major fermentation products in SFM, whereas in SFM minus HCO-3 or haemin, lactate was the major product and only small quantities of other acids accumulated.
化脓棒状杆菌在无血清培养基（SFM）中需氧或厌氧培养时，其生长及酸性代谢产物相似。这表明，即使在有氧生长条件下，化脓棒状杆菌主要通过发酵代谢获取生长所需能量。在不含葡萄糖的SFM中，生长产量降低90%；在不含NaHCO₃的SFM中，降低50%；在不含酵母提取物的SFM中，降低90%；在不含胰蛋白酶和酵母提取物的SFM中，降低100%；在不含血红素或胰蛋白酶的SFM中，降低30%。当用已知的氨基酸、维生素和核酸碱基混合物替代SFM中的胰蛋白酶和酵母提取物时，无法检测到生长；在后者培养基中添加肽源（如胰蛋白酶或酪蛋白胨）可支持该菌良好生长。当从SFM中省略NaHCO₃并严格排除培养基中的溶解CO₂时，无法检测到生长，这表明化脓棒状杆菌生长绝对需要CO₂。琥珀酸、甲酸和乙酸是SFM中的主要发酵产物，而在不含HCO₃⁻或血红素的SFM中，乳酸是主要产物，只有少量其他酸积累。
