Bertoni-Freddari C, Giuli C, Pieri C
Arch Gerontol Geriatr. 1982 Dec;1(4):365-73. doi: 10.1016/0167-4943(82)90036-x.
The cerebellar glomerulus was studied by electron microscopic morphometry in female Wistar rats. Age-dependent alterations have been revealed from 3 to 28 mth of age, and the effect of centrophenoxine (CPH) was analyzed in two different patterns of administration. First, 27-mth-old rats were treated daily for 6 wk (acute treatment), and second, 18-mth-old rats were treated 3 times per week for 5 months (chronic treatment). The dose was 100 mg CPH/kg body weight, injected intraperitoneally. The surface density (SV), the numerical density (NV) and the average length (L) of the synaptic junctions were calculated from data obtained on ethanol-phosphotungstic acid stained ultrathin sections. An age-dependent reduction of SV and NV of the synaptic contact zones was found, and the L increased in the oldest animals. CPH-treatment resulted in a marked increase of SV in both types of application, whereas the other two parameters behaved differently in the two groups. The chronic treatment resulted in a significant slowing down of the decrease of NV, whereas L remained invariate. On the contrary, the acute treatment increased L but did not alter significantly NV. The results and the differences between the treatment types are discussed in terms of synaptic plasticity and are interpreted as different manifestations of the same reactive synaptogenetic process.
通过电子显微镜形态计量学对雌性Wistar大鼠的小脑小球进行了研究。揭示了3至28月龄大鼠的年龄依赖性变化，并分析了在两种不同给药模式下中枢酚（CPH）的作用。首先，对27月龄大鼠每天治疗6周（急性治疗），其次，对18月龄大鼠每周治疗3次，持续5个月（慢性治疗）。剂量为100 mg CPH/ kg体重，腹腔注射。根据在乙醇-磷钨酸染色超薄切片上获得的数据计算突触连接的表面密度（SV）、数值密度（NV）和平均长度（L）。发现突触接触区的SV和NV存在年龄依赖性降低，而最年长动物的L增加。CPH治疗在两种应用类型中均导致SV显著增加，而另外两个参数在两组中的表现不同。慢性治疗导致NV降低的显著减缓，而L保持不变。相反，急性治疗增加了L，但未显著改变NV。根据突触可塑性讨论了结果和治疗类型之间的差异，并解释为同一反应性突触发生过程的不同表现。
