Muirhead K A, Schmitt T C, Muirhead A R
Cytometry. 1983 Jan;3(4):251-6. doi: 10.1002/cyto.990030404.
Logarithmic amplifiers are useful in accumulating flow cytometric data with a large dynamic range. However, quantitative comparison of fluorescence intensities for different samples or different subpopulations within a sample is simplified by the conversion of data from log space back to linear space. A method is described in which fluorescent polystyrene spheres of differing intensities are used to construct a calibration curve for the logarithmic intensity scale. This allows calculation of relative linear intensity for each channel of the logarithmically accumulated data and determination of linear fluorescence means and coefficients of variation for comparative purposes. Fluorescent spheres of appropriate intensity may also be used as internal standards to monitor instrument and/or stain stability for samples accumulated using logarithmic amplifiers.
对数放大器在积累具有大动态范围的流式细胞术数据时很有用。然而，通过将数据从对数空间转换回线性空间，可简化对不同样本或样本内不同亚群的荧光强度进行定量比较。本文描述了一种方法，其中使用不同强度的荧光聚苯乙烯微球构建对数强度标度的校准曲线。这使得能够计算对数积累数据每个通道的相对线性强度，并确定线性荧光平均值和变异系数以用于比较目的。适当强度的荧光微球也可用作内标，以监测使用对数放大器积累的样本的仪器和/或染色稳定性。
