Hellström I, Garrigues H J, Cabasco L, Mosely G H, Brown J P, Hellström K E
J Immunol. 1983 Mar;130(3):1467-72.
Hybridomas were generated by fusing SP2/0 mouse myeloma cells with spleen cells from mice that had been immunized with cultured human melanoma cells. One of the hybridomas secreted a monoclonal IgG1 antibody, 48.7, which binds to a cell surface antigen of cells from human melanomas and compound nevi. The presence of the target antigen in vivo was demonstrated immunohistologically by staining frozen sections of primary and metastatic melanoma by the peroxidase anti-peroxidase technique. Weak staining of some blood vessel cells was also seen, but other normal cells, including skin melanocytes, were unstained, as were cells from other tumor types. Antibody 48.7 immunoprecipitated polypeptides with apparent m.w. on sodium dodecyl sulfate-polyacrylamide gel electrophoresis of 250,000 and greater than 400,000.
通过将SP2/0小鼠骨髓瘤细胞与用培养的人黑色素瘤细胞免疫的小鼠的脾细胞融合，产生了杂交瘤。其中一种杂交瘤分泌一种单克隆IgG1抗体48.7，它与来自人黑色素瘤和复合痣的细胞表面抗原结合。通过过氧化物酶抗过氧化物酶技术对原发性和转移性黑色素瘤的冰冻切片进行染色，免疫组织化学证明了体内靶抗原的存在。还观察到一些血管细胞有弱阳性染色，但其他正常细胞，包括皮肤黑素细胞，均未染色，其他肿瘤类型的细胞也是如此。抗体48.7在十二烷基硫酸钠-聚丙烯酰胺凝胶电泳上免疫沉淀出表观分子量为250,000和大于400,000的多肽。
