Malik G M, Canawati H N, Keyser A J, Ibrahim M Z, Montgomerie J Z
J Infect Dis. 1983 Jan;147(1):161. doi: 10.1093/infdis/147.1.161.
A number of indirect methods have been developed to determine the site of urinary tract infection, including the measurement of LDH in urine [1]. Although LDH has been thought to be from the kidneys, it has also been noted that leukocytes could contribute LDH isoenzymes 4 and 5 [2]. Seventeen patients with injured spinal cords and significant bacteriuria were included in this study. Urine specimens obtained by urethral catheter were cultured, and PMNLs identified with Sternheimer-Malbin stain were counted in a hemacytometer. A positive test for antibody-coated bacteria and the lack of patient response to five to 10 days of antibiotic therapy were used as an indication of upper urinary tract infection. Levels of LDH isoenzymes 4 and 5 (cathodal) correlated with the number of PMNLs in the urine (r = 0.63, P less than 0.01). There was no correlation of PMNLs with LDH isoenzymes 1 and 2 (r = 0.18). In addition, there was no correlation of LDH isoenzymes 4 and 5 with the level of urinary tract infection. These results suggest that the PMNLs in the urine are the source of the LDH isoenzymes 4 and 5.
已经开发了许多间接方法来确定尿路感染的部位，包括测量尿液中的乳酸脱氢酶（LDH）[1]。尽管一直认为LDH来自肾脏，但也有人指出白细胞可能会产生LDH同工酶4和5[2]。本研究纳入了17例脊髓损伤且有明显菌尿的患者。对通过导尿管获取的尿液标本进行培养，并在血细胞计数器中对用斯特恩海默-马尔宾染色鉴定的中性粒细胞进行计数。抗体包被细菌检测呈阳性以及患者对5至10天抗生素治疗无反应被用作上尿路感染的指标。LDH同工酶4和5（阴极）的水平与尿液中的中性粒细胞数量相关（r = 0.63，P小于0.01）。中性粒细胞与LDH同工酶1和2无相关性（r = 0.18）。此外，LDH同工酶4和5与尿路感染的程度无相关性。这些结果表明尿液中的中性粒细胞是LDH同工酶4和5的来源。
