Winocour P D, Kinlough-Rathbone R L, Perry D W, Rand M L, Packham M A, Mustard J F
J Lab Clin Med. 1983 Feb;101(2):175-82.
Platelet survival is shortened in experimental animals in which indwelling aortic catheters have been placed. We have examined the properties of platelets harvested from rats on day 1 or day 6 after insertion of indwelling aortic catheters. Platelets taken from these animals at 6 days (but not at 1 day) survived in normal rats or in rats with indwelling catheters for a significantly longer time than platelets from sham-operated rats. The catheters caused a persistent fall in the platelet count, an increase in the proportion of platelets in the most dense fraction after separation on discontinuous Stractan density gradients, and a decrease in mean sialic acid and protein per 10(9) platelets, but not change in modal size as determined with a Coulter Counter Channelyzer. No significant differences in the sensitivity to aggregating and release-inducing agents (ADP, thrombin, or collagen) were observed. It seems likely that chronic damage of the vessel wall shortened platelet survival and increased platelet turnover, although the increase in platelet production was not sufficient to maintain the platelet counts at the same values as in the sham-operated animals. The prolonged survival times in recipient rats of platelets from rats with indwelling aortic catheters indicate that a population of predominantly young platelets exists in these animals. Their increased density supports this conclusion.
在已植入主动脉留置导管的实验动物中，血小板存活时间缩短。我们研究了在植入主动脉留置导管后第1天或第6天从大鼠采集的血小板的特性。从这些动物在第6天（而非第1天）采集的血小板，在正常大鼠或有留置导管的大鼠体内存活的时间，比假手术大鼠的血小板显著更长。导管导致血小板计数持续下降，在不连续的Stractan密度梯度分离后，最致密部分的血小板比例增加，每10⁹个血小板的平均唾液酸和蛋白质含量减少，但用库尔特计数器通道分析仪测定的模态大小没有变化。未观察到对聚集和释放诱导剂（ADP、凝血酶或胶原）的敏感性有显著差异。血管壁的慢性损伤似乎缩短了血小板存活时间并增加了血小板周转率，尽管血小板生成的增加不足以使血小板计数维持在与假手术动物相同的水平。来自有主动脉留置导管大鼠的血小板在受体大鼠中的存活时间延长，表明这些动物中存在主要为年轻血小板的群体。它们密度的增加支持了这一结论。
