母马体内一种新的妊娠蛋白的免疫化学证明。

Immunochemical demonstration of a new pregnancy protein in the mare.

作者信息

Gidley-Baird A A, Teisner B, Hau J, Grudzinskas J G

出版信息

J Reprod Fertil. 1983 Jan;67(1):129-32. doi: 10.1530/jrf.0.0670129.

DOI:10.1530/jrf.0.0670129

Abstract

An antiserum against the serum of a pregnant mare was absorbed with stallion serum. This antiserum then gave two precipitates in crossed immunoelectrophoresis with serum from pregnant mares as the antigen. The two precipitates exhibited beta-1 and alpha-2 electrophoretic mobility. Identity was demonstrated between the alpha-2 mobile protein and PMSG. The absorbed antiserum inhibited the biological action of the PMSG preparation when tested in mouse ovarian weight assays. The beta-1 mobile protein was not detected in the serum from non-pregnant mares, stallions or geldings and was detected earlier in pregnancy (Day 30) than was PMSG (Day 42).

摘要

一种抗孕马血清的抗血清用雄马血清吸收。然后，以孕马血清为抗原，该抗血清在交叉免疫电泳中产生了两种沉淀。这两种沉淀表现出β-1和α-2电泳迁移率。已证实α-2迁移蛋白与孕马血清促性腺激素（PMSG）相同。在小鼠卵巢重量测定试验中，吸收后的抗血清抑制了PMSG制剂的生物学活性。在未孕母马、雄马或去势雄马的血清中未检测到β-1迁移蛋白，且其在妊娠早期（第30天）比PMSG（第42天）更早被检测到。