Royle G T, Wolfe R R, Burke J F
J Surg Res. 1983 Feb;34(2):187-93. doi: 10.1016/0022-4804(83)90059-8.
Isotopes have become the best means for investigating glucose kinetics in vivo. With the recent greater availability of stable isotopes there has developed a need to understand how data may be obtained from the use of both radioactive and stable glucose tracers. Described for the nonexpert is the calculation of glucose appearance and disappearance, clearance and oxidation using both stable and radioactive glucose isotopes, administered both by bolus and primed constant infusion and under both steady and nonsteady state conditions. Other substrates may be studied using similar methodology. The use of stable substrate isotopes will be an expanding field of metabolic research in man.
同位素已成为体内研究葡萄糖动力学的最佳手段。随着近期稳定同位素的可得性提高，人们开始需要了解如何通过使用放射性和稳定葡萄糖示踪剂来获取数据。本文为非专业人士描述了使用稳定和放射性葡萄糖同位素，通过单次推注和预充恒速输注，在稳态和非稳态条件下计算葡萄糖的出现和消失、清除率和氧化率的方法。其他底物也可采用类似方法进行研究。稳定底物同位素的应用将成为人体代谢研究中一个不断拓展的领域。
