Royle G T, Wolfe R R, Burke J F
Ann R Coll Surg Engl. 1981 Nov;63(6):415-9.
Studies of dynamic aspects of substrate metabolism in patients can be made with a variety of techniques. Methods include indirect calorimetry, substrate load tests, measurement of arteriovenous differences with use of the Fick principle, and isotope infusions. Recent developments in stable isotope methodology have made available safe techniques for accurate measurement of the turnover of plasma substrates in patients. The use of deuterated, 13C, and 15N isotopes enables the turnover of several substrates to be measured at the same time. It is hoped that the primed constant infusion of stable isotopes combined with indirect calorimetry will lead to a greater understanding of metabolism in ill patients.
可以采用多种技术对患者底物代谢的动态方面进行研究。方法包括间接测热法、底物负荷试验、运用菲克原理测量动静脉差值以及同位素输注。稳定同位素方法学的最新进展已提供了安全的技术，用于准确测量患者血浆底物的周转率。使用氘代、13C和15N同位素能够同时测量几种底物的周转率。人们希望稳定同位素的单次静脉恒速输注与间接测热法相结合，将有助于更深入地了解患病患者的代谢情况。
