Preisler H D
Oncology. 1983;40(1):67-71. doi: 10.1159/000225694.
Centrifugal elutriation has been adapted for use in obtaining highly enriched populations of leukemic myeloblasts and normal appearing lymphocytes from RFM/UN mice with myeloid leukemia. The myeloblasts were functionally intact as evidenced by their high cloning efficiency in vitro and malignant potential in vivo. The lymphocyte-enriched subpopulation had a low thymidine labeling index, and cloning efficiency in vitro, and was only marginally malignant in vivo indicating minimal contamination by leukemic myeloblasts. In preterminal leukemic mice the proportion of cycling leukemic cells remained high with a labeling index of 30-50%.
离心淘析已被应用于从患有髓性白血病的RFM/UN小鼠中获取高度富集的白血病成髓细胞群和外观正常的淋巴细胞。成髓细胞功能完整，这在其体外高克隆效率和体内恶性潜能中得到了证明。富含淋巴细胞的亚群具有低胸苷标记指数和体外克隆效率，并且在体内仅具有轻微的恶性，表明白血病成髓细胞的污染最小。在终末期白血病小鼠中，循环白血病细胞的比例仍然很高，标记指数为30-50%。
