Preisler H D
Antibiot Chemother (1971). 1980;28:130-6. doi: 10.1159/000386071.
Neoplastic cells are frequently intermingled with normal cells in vivo in man and in laboratory animals. In addition, tumor cells frequently consist of functionally distinct cell subpopulations. We have used both centrifugal elutriation and electronic cell sorting to separate leukemic cells from nonleukemic hematopoietic cells and to separate kinetically distinct subpopulations of leukemia cells. In addition, methods have been developed to use pulse cytophotometry to detect the interaction of the anthracyclines with leukemic cells during exposure, both in vitro and in vivo.
在人类和实验动物体内，肿瘤细胞常常与正常细胞相互混杂。此外，肿瘤细胞通常由功能不同的细胞亚群组成。我们已运用离心淘析法和电子细胞分选法，将白血病细胞与非白血病造血细胞分离，并分离出白血病细胞动力学上不同的亚群。此外，还开发了一些方法，利用脉冲细胞光度测定法在体外和体内检测蒽环类药物在接触过程中与白血病细胞的相互作用。
