Counsell R E, Korn N, Pohland R C, Schwendner S W, Seevers R H
Biochim Biophys Acta. 1983 Mar 1;750(3):497-503. doi: 10.1016/0005-2760(83)90190-x.
Radioiodinated cholesteryl oleate (125I-CO) was found to associate rapidly with plasma lipoproteins following intravenous administration to rats. The high-density lipoprotein (HDL) fraction was observed to contain the highest amount of radioiodinated ester. Isolation and purification of this HDL fraction (125I-CO-HDL) and subsequent administration to rats demonstrated a plasma clearance similar to that previously observed for HDL labeled by direct iodination. Moreover, the concentration of radioactivity appearing in the adrenal cortex and ovary 0.5 h after intravenous administration of 125I-CO-HDL was greater than that observed after administration of 125I-CO, and the uptake of radioactivity by these tissues was considerably greater in hypolipidemic rats. These findings are consistent with existing knowledge relating to the metabolic fate of HDL and radioiodinated cholesterol derivatives in the rat, and suggest that radioiodinated cholesteryl esters may become useful probes for labeling lipoproteins.
给大鼠静脉注射放射性碘化胆固醇油酸酯（125I-CO）后，发现其能迅速与血浆脂蛋白结合。观察到高密度脂蛋白（HDL）部分所含放射性碘化酯的量最高。分离并纯化该HDL部分（125I-CO-HDL），随后给大鼠注射，结果显示其血浆清除率与先前观察到的直接碘化标记的HDL相似。此外，静脉注射125I-CO-HDL后0.5小时，肾上腺皮质和卵巢中出现的放射性浓度高于注射125I-CO后观察到的浓度，并且在低脂血症大鼠中，这些组织对放射性的摄取量明显更大。这些发现与大鼠中HDL和放射性碘化胆固醇衍生物的代谢命运的现有知识一致，并表明放射性碘化胆固醇酯可能成为标记脂蛋白的有用探针。
