De Togni P, Della Bianca V, Bellavite P, Grzeskowiak M, Rossi F
Biochim Biophys Acta. 1983 Feb 22;755(3):506-13. doi: 10.1016/0304-4165(83)90256-8.
Studies were carried out on the mechanism responsible for the enhancement of the respiratory and secretory responses to N-formylmethionylleucylphenylalanine (fMet-Leu-Phe) exhibited by human neutrophils suspended in Na+-free, high-K+ buffered solution. The results demonstrate that: (a) the variation of Na+ concentration in the suspending solution induces in human neutrophils a marked modification of the recognition apparatus for the chemotactic peptide fMet-Leu-Phe, the lack of or low concentration of this ion increasing the number of the receptors and their specific affinity for the ligand; (b) the greater respiratory burst and secretion induced by fMet-Leu-Phe in human neutrophils suspended in Na+-free, high-K+ medium are due to the increased formation of receptor-ligand complexes at the cell membrane; (c) the greater respiratory response is partially due also to a higher efficiency of these receptor-ligand complexes. The molecular mechanism by which Na+ exerts a regulative role on the properties of the recognition apparatus for the chemotactic peptide and its possible significance are discussed.
对悬浮于无钠、高钾缓冲溶液中的人中性粒细胞对N-甲酰甲硫氨酰亮氨酰苯丙氨酸（fMet-Leu-Phe）的呼吸和分泌反应增强的机制进行了研究。结果表明：（a）悬浮溶液中Na+浓度的变化导致人中性粒细胞对趋化肽fMet-Leu-Phe的识别装置发生显著改变，该离子的缺乏或低浓度会增加受体数量及其对配体的特异性亲和力；（b）在无钠、高钾培养基中悬浮的人中性粒细胞中，fMet-Leu-Phe诱导的更大的呼吸爆发和分泌是由于细胞膜上受体-配体复合物形成增加；（c）更大的呼吸反应部分也归因于这些受体-配体复合物的更高效率。讨论了Na+对趋化肽识别装置特性发挥调节作用的分子机制及其可能的意义。
