Viazova E P, Azhigirova M A, Fetisova L V, Khachatur'ian A A, Rozenberg G Ia
Biull Eksp Biol Med. 1983 Feb;95(2):50-2.
Oxygen-dissociation properties and some aspects of allosteric regulation of bovine hemoglobin (Hb) and its polymerized form (HbP) have been examined. It has been shown that bovine HbP as well as bovine Hb does not require the presence of organic phosphates for normal functioning and has gas transport characteristics which are similar to those of the whole human blood. Pyridoxal-5'-phosphate (PP) works as an allosteric regulator of bovine HbP only in the absence of Cl- ions and vice versa. However, in covalent binding of PP to HbP both the effectors (PP and Cl- ions) can work simultaneously and independently. Thus, by varying the Hb/PP ratio it is possible to prepare HbP solutions with preset P50 values within the range 30-55 mm Hg. The results can be used in simulation of gas transport characteristics of artificial oxygen carriers.
对牛血红蛋白（Hb）及其聚合形式（HbP）的氧解离特性和变构调节的某些方面进行了研究。结果表明，牛HbP和牛Hb在正常功能发挥时不需要有机磷酸盐的存在，并且具有与全人类血液相似的气体运输特性。仅在不存在Cl-离子的情况下，磷酸吡哆醛-5'-磷酸（PP）才作为牛HbP的变构调节剂起作用，反之亦然。然而，在PP与HbP的共价结合中，两种效应物（PP和Cl-离子）可以同时且独立地起作用。因此，通过改变Hb/PP比例，可以制备出P50值预设在30-55 mmHg范围内的HbP溶液。这些结果可用于模拟人工氧载体的气体运输特性。
