Aisenberg A C, Wilkes B M, Harris N L
Blood. 1983 Mar;61(3):469-75.
The cell lineage of suspensions prepared from 85 non-Hodgkin's lymphomas was investigated with a panel of 10 monoclonal antibodies and conventional surface marker techniques. Surface immunoglobulin, assessed with specific heteroantisera, proved to be the most useful characteristic and defined the clonal character and B-cell lineage of 63 specimens: almost all nodular lymphocytic (21 of 22) and diffuse lymphocytic (11 of 13) lymphomas, most diffuse histiocytic (29 of 33) and diffuse mixed (2 of 2) lymphomas, and a few nodular mixed (2 of 12) and nodular histiocytic (0 of 3) lymphomas. Monoclonal antibodies provided useful ancillary surface marker criteria. Thus, positivity with OKT1 (which detects both thymic and peripheral T cells) in the absence of reactivity with monoclonal antisera, which detect only peripheral T cells (OKT3, OKT4, OKT8, and OKT11), was seen only in diffuse lymphocytic lymphoma of B lineage. Ia-like antigen could be demonstrated in all B-cell lymphocytic lymphomas and most B-cell diffuse histiocytic lymphomas. Approximately one-half of diffuse histiocytic lymphomas also reacted with OKT9, which detects the transferrin receptor, while few lymph nodes involved by other conditions displayed this reactivity. Most diffuse histiocytic lymphomas and many non-Hodgkin's lymphomas of other subtypes reacted with OKT10, an antiserum that detects an antigen on replicating lymphoid cells. The lineage of approximately one-fourth of the lymphoma suspensions was not resolved conclusively: In most of these, T lymphocytes predominated with a normal proportion of inducer-helper (OKT4) and cytotoxic-suppressor (OKT8) cells. The inability to establish the clonal character of T-cell proliferation in cell suspensions remains an obstacle to completely defining the lineage of non-Hodgkin's lymphomas.
使用一组10种单克隆抗体和传统表面标志物技术，对从85例非霍奇金淋巴瘤制备的细胞悬液的细胞谱系进行了研究。用特异性异种抗血清评估的表面免疫球蛋白被证明是最有用的特征，并确定了63个标本的克隆特征和B细胞谱系：几乎所有结节性淋巴细胞性（22例中的21例）和弥漫性淋巴细胞性（13例中的11例）淋巴瘤、大多数弥漫性组织细胞性（33例中的29例）和弥漫性混合性（2例中的2例）淋巴瘤，以及少数结节性混合性（12例中的2例）和结节性组织细胞性（3例中的0例）淋巴瘤。单克隆抗体提供了有用的辅助表面标志物标准。因此，仅在B谱系的弥漫性淋巴细胞性淋巴瘤中可见，在与仅检测外周T细胞的单克隆抗血清（OKT3、OKT4、OKT8和OKT11）无反应的情况下，OKT1（检测胸腺和外周T细胞）呈阳性。Ia样抗原可在所有B细胞淋巴细胞性淋巴瘤和大多数B细胞弥漫性组织细胞性淋巴瘤中显示。大约一半的弥漫性组织细胞性淋巴瘤也与检测转铁蛋白受体的OKT9反应，而其他情况累及的少数淋巴结显示这种反应性。大多数弥漫性组织细胞性淋巴瘤和许多其他亚型的非霍奇金淋巴瘤与OKT10反应，OKT10是一种检测复制淋巴样细胞上抗原的抗血清。大约四分之一的淋巴瘤悬液的谱系未能最终确定：在大多数这些悬液中，T淋巴细胞占主导，诱导辅助细胞（OKT4）和细胞毒性抑制细胞（OKT8）比例正常。在细胞悬液中无法确定T细胞增殖的克隆特征仍然是完全确定非霍奇金淋巴瘤谱系的一个障碍。
