Knowles D M, Dodson L, Burke J S, Wang J M, Bonetti F, Pelicci P G, Flug F, Dalla-Favera R, Wang C Y
Am J Pathol. 1985 Sep;120(3):356-70.
The authors performed immunophenotypic, functional, and molecular analysis of the neoplastic cells from 20 cases of SIg-, E-("null-cell") non-Hodgkin's lymphoma (NHL) in order to determine their lineage, better define this category of NHL, and evaluate the lineage specificity of selected phenotypic markers and the individual and collective utility of these approaches. They assigned 4 cases to the T-cell lineage, and 15 cases to the B-cell lineage, and 1 case remained indeterminant on the basis of immunophenotypic analysis. The cells from 2 cases assigned to the T-cell lineage expressed unusual phenotypes, but their T-cell derivation was confirmed by the demonstration of helper function in vitro. The 15 cases assigned to the B-cell lineage expressed a variety of B-cell-associated antigens, consistent with various stages of B-cell differentiation. Monoclonal antibodies OKT3, OKT4, OKT6, and OKT8 exhibited T-cell lineage restriction; and monoclonal antibodies OKB2, BL1, and B1 exhibited B-cell lineage restriction. Ia, TdT, cALLa, OKT9, and OKT10 exhibited lineage infidelity. Southern blot analysis for immunoglobulin heavy chain gene rearrangements confirmed 18 of the 19 lineage assignments made by immunophenotypic analysis and suggested that the 1 case of indeterminate phenotype was a B-cell neoplasm. One T-cell (OKT3+, T4+) neoplasm exhibited rearranged immunoglobulin heavy chain genes. Thus, neither immunophenotypic analysis nor the demonstration of rearranged immunoglobulin heavy chain genes alone permitted the satisfactory lineage assignment of every case of SIg-, E- NHL. However, combined immunophenotypic, functional, and genotypic analysis allowed us to assign every SIg-, E-NHL to the B- or T-cell lineage and to demonstrate that truly "null-cell" NHLs are probably very uncommon.
作者对20例表面免疫球蛋白阴性、E阴性（“无标记细胞”）非霍奇金淋巴瘤（NHL）的肿瘤细胞进行了免疫表型、功能和分子分析，以确定其细胞系，更好地界定此类NHL，并评估所选表型标志物的细胞系特异性以及这些方法的个体和综合效用。根据免疫表型分析，他们将4例归为T细胞系，15例归为B细胞系，1例仍无法确定。2例归为T细胞系的细胞表现出异常表型，但通过体外辅助功能的证明证实了它们的T细胞来源。归为B细胞系的15例表达了多种与B细胞相关的抗原，与B细胞分化的不同阶段一致。单克隆抗体OKT3、OKT4、OKT6和OKT8表现出T细胞系限制性；单克隆抗体OKB2、BL1和B1表现出B细胞系限制性。Ia、TdT、cALLa、OKT9和OKT10表现出细胞系不忠实性。免疫球蛋白重链基因重排的Southern印迹分析证实了免疫表型分析做出的19例细胞系分类中的18例，并提示1例表型不确定的病例是B细胞肿瘤。1例T细胞（OKT3 +、T4 +）肿瘤表现出免疫球蛋白重链基因重排。因此，单独的免疫表型分析或免疫球蛋白重链基因重排的证明均不能使每例表面免疫球蛋白阴性、E阴性NHL获得令人满意的细胞系分类。然而，联合免疫表型、功能和基因分析使我们能够将每例表面免疫球蛋白阴性、E阴性NHL归为B或T细胞系，并证明真正的“无标记细胞”NHL可能非常罕见。
